异黄酮辅剂对解冻精子特征的影响

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

本研究评价了使用异黄酮作为辅剂的解冻剂对解冻精子精液参数的影响。我们分析了辅剂在解冻过程中对精子活力、精子获能能力（膜脂功能紊乱）、活性氧生成、精子核浓缩及DNA损害的影响。根据初步的数据，辅剂可能会改善解冻过程，减少细胞损伤。我们已经证实异黄酮在精子解冻过程中有抗氧化作用、能够减少活性氧的生成，有利于精子活力轻微提高，降低膜脂功能紊乱和由冷冻而引起的DNA破坏。结果显示异黄酮作为辅剂有助于提高精子功能，这对于辅助生育中使用解冻精子很有意义。我们的发现有必要进一步研究来确证并评价临床应用的可能性。

In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation （membrane lipid disorder）, reactive oxygen species （ROS） generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreservation process and reduce the cellular damage. We have confirmed that the isoflavone genistein （10 μmol L-1） has antioxidant properties on the frozen-thawed spermatozoa. This results in a decreased ROS production that shows a slight improvement in the sperm motility, and decreases the membrane lipid disorder and DNA damage caused by cryopreservation. These results suggest an effect of genistein on sperm functionality that could be of interest for assisted reproduction treatments using frozen- thawed human spermatozoa, but further studies will be necessary to confirm our findings and to evaluate the possible clinical applications.