微波辅助酸水解法制备丝素肽的研究

Research on Preparation of Silk Fibroin Peptide with Microwave-assisted Hydrolysis

[目的]建立并优化快捷、高效制备丝素肽的微波辅助酸水解工艺。[方法]以家蚕废丝为原料,比较在碱液脱胶、磷酸酸解、石灰水沉淀除盐、活性炭脱色的工艺流程中酸水解的优化方案,并探讨亚硫酸和磷酸混合酸解对后继脱色工段的影响。[结果]微波辅助磷酸水解丝素蛋白最佳工艺条件为:磷酸浓度85%,温度70℃,功率150W,酸料比4∶1,水解90min,产品回收率达到69.0%,比磷酸水浴水解方案高约15%,且节省了4～5h的水解时间;在微波辅助水解条件下,亚硫酸和磷酸混合酸解比单独用磷酸酸解所得水解液颜色浅,色值可降低65.46%。[结论]对于丝肽的酸水解法制备,微波辅助能大大降低能耗,节省成本;混合酸酸水解法能降低酸解液色值。

[Objective] The study aimed to establish a effective and fast preparation method for silk fibroin peptide with microwave-assisted hydrolysis.[Method] Silkworm waste silk was utilized to prepare peptide by degumming,hydrolyzing silk fibroin with phosphoric,desalting with precipitation separation method,decolorizing with activated carbon.The influence on liquid color was discussed from hydrolysis of mixed acid.[Result] The optimum hydrolysis conditions for fibroin peptide with microwave-assisted hydrolysis were as follows：the phosphoric acid concentration of 85%,the hydrolyzing temperature of 70 ℃,reaction time of 90 min,microwave power of 150 W,liquid-solid ratio of 4∶1,the recovery rate was above 69%,which was more 15% than traditional method and less 4 h.The hydrolysis of mixed acid reduced the value of 65.46% on liquid color.[Conclusion] This method was rapid,reliable and the method of mixed acid reduced the color value.