高压氧和5-氟尿嘧啶联合作用对鼻咽癌细胞增殖与侵袭迁移的影响

Effect of the combination of hyperbaric oxygen and 5-fluorouracil on the proliferation and metastasis of nasopharyngeal carcinoma CNE-2Z cell line

目的:从细胞水平探讨高压氧或(和)5-氟尿嘧啶(5-FU)处理对鼻咽癌CNE-2Z细胞增殖与侵袭转移的影响及其影响机制。方法:将实验用人鼻咽癌CNE2Z细胞分为对照组(A组)、5-FU组(B组)、高压氧组(C组)、5-FU加高压氧组(D组)4组。采用MTT比色法观察各组处理24、48和72h后对鼻咽癌CNE-2Z细胞增殖的抑制作用;采用Transwell小室分析法评价各组处理对鼻咽癌细胞侵袭、迁移相关能力的影响;采用细胞免疫化学染色法检测各组鼻咽癌细胞MMP-9、VEGF的表达水平。结果:C组处理48h和72h后鼻咽癌细胞增殖抑制A值与A组比较均差异有统计学意义(均P<0.01),D组处理仅48h后分别与A、B、C组比较差异有统计学意义(P<0.05);C组鼻咽癌细胞侵袭迁移结果和MMP-9和VEGF表达的平均值与A组比较差异均无统计学意义(P>0.05),虽D组鼻咽癌细胞侵袭、迁移结果和MMP-9和VEGF表达的平均A值与A组比较均差异有统计学意义(P<0.01),但与C组比较均差异无统计学意义(P>0.05)。结论:单纯高压氧处理48h和72h后均能明显抑制人鼻咽癌细胞的增殖,但高压氧和5-FU联合处理仅在处理48h后才能更明显抑制人鼻咽癌细胞的增殖;单纯高压氧处理不能明显降低鼻咽癌细胞MMP-9、VEGF的高表达,也不能抑制鼻咽癌细胞的侵袭迁移能力;并且高压氧联合5-FU处理也不能较单纯5-FU处理更进一步降低鼻咽癌细胞MMP-9、VEGF的表达和抑制鼻咽癌细胞侵袭与迁移。

Objective：To investigate the effect and the possible influencial mechanisms of hyperbaric oxygen（HBO） and 5-fluorouracil（5-FU） on the proliferation and metastasis in human nasopharyngeal carcinoma （NPC） CNE2Z cell line.Method：NPC CNE2Z cell line were divided into 4 groups randomly： group A： control group; group B：5-FU group;group C： HBO group; group D：5-FU and HBO group. The inhibition effect of proliferation in CNE2Z cells of all groups through 24 h、48 h and 72 h disposal was detected by MTT reduction assay; Transwell chamber assay was performed to determine the effect of HBO and（or） 5-FU on the metastasis of the CNE2Z cells; The MMP-9、VEGF expression in CNE2Z cells of all groups were detected by SP immunocytochemical stain and observe the expressed image by micro.Result：There were statistical difference on the inhibition effect of proliferation in C group and A group after 48 h and 72 h disposal（P0.01） and between A、B、C group and D group only after 48 h disposal（P0.05）;There were not statistical difference on the effect of metastasis in C group and A group（P0.05） and between B、C group and D group（P0.05）; Average optical of the MMP-9、VEGF expression were not statistically significant difference in C group and A group（P0.05） and between C group and D group（P0.05）.Conclusion：Simple HBO disposal after 48 h and 72 h could inhibit the proliferation potential of NPC CNE2Z cell line, but the combination of HBO and 5-FU only after 48 h disposal could all the more inhibit the proliferation potential. Simple HBO disposal couldn＇t decrease the MMP-9 and VEGF high-expression and inhibit the metastasis potential in human NPC CNE2Z cell line, the combination of HBO and 5-FU disposal also couldn＇t further decreased the MMP-9 and VEGF high-expression and inhibited the metastasis potential.