基因芯片筛选喉鳞状上皮细胞癌相关基因的实验研究

A verification study on the genes associated with laryngeal squamous cell carcinoma by cDNA microarray

目的:进行喉鳞状上皮细胞癌相关基因的验证研究,为喉鳞状上皮细胞癌相关基因的进一步研究打下基础。方法:在基因芯片筛选结果中挑出部分基因(SENP1、CD109、Lamininα2、Lamininα3),应用RT-PCR、Western blot进一步检测这些基因在12例喉鳞状上皮细胞癌及其癌旁正常组织中mRNA和蛋白水平的表达。结果:RT-PCR结果显示SENP1、CD109、Lamininα3基因在喉鳞状上皮细胞癌组织中表达上调,Lamininα2基因表达下调。Westernblot结果显示SENP1、CD109蛋白在喉鳞状细胞癌组织中的表达明显高于对应的癌旁正常组织。结论:SENP1、CD109、Lamininα2、Lamininα3可能与喉鳞状上皮细胞癌的发生、发展相关,这对从分子水平研究喉癌的发病机制提供了有益的线索。

Objective：Our previous study revealed many genes differentially expressed in human laryngeal squamous cell carcinoma tissues（LSCC） and related adjacent normal tissues. We verificated the differentiated expressions of target genes, possibly related to LSCC. And these results can be foudations of further studys of these target genes function.Method：SENP1, CD109, Lamininα2, Lamininα3 were selected according to the cDNA microarray results. The expression of these genes mRNA and protein were detected by RT-PCR and Western blot in 12 cases of LSCC and related adjacent normal tissues.Result：The mRNA expression of SENP1, CD109 and Lamininα3 were significantly higher while Lamininα2 were significantly lower in LSCC tissues than in corresponding adjacent normal tissues by Semiquantitative RT-PCR. Western blot analysis revealed SENP1, CD109 protein expression were significantly higher in LSCC tissues than in corresponding adjacent normal tissues.Conclusion：SENP1, CD109, Lamininα2 and Lamininα3 may correlated with tumorigenesis and development of LSCC and can provide beneficial clue for study pathogenesis of LSCC in molecular level.