栀子总环烯醚萜苷对大鼠脑出血AQP4和MMP-2/9表达的影响

Effects of total iridoid glycosides in Gardenia jasminoides on the expression of AQP4 and MMP-2/9 in perihematomal brain regions of rats

目的:探讨栀子总环烯醚萜苷对大鼠脑出血AQP4和MMP-2/9表达的影响。方法:SD大鼠随机分为假手术组、模型组和栀子总环烯醚萜苷(30mg/kg)治疗组,采用自体血注入诱发大鼠脑出血模型,术后24、48、72和120h时间点各组各取5只大鼠脑组织标本,采用干湿重法、免疫组织化学染色、逆转录聚合酶链反应技术分别测定脑含水量、AQP4蛋白和AQP4mRNA的表达、MMP-2和MMP-9表达。结果:与模型组比较,治疗组大鼠在各时间点脑含水量减少(P<0.01),AQP4蛋白和AQP4mRNA表达降低(P<0.05),术后72～120h时间点MMP-2和MMP-9表达水平低(P<0.05～P<0.01)。结论:栀子总环烯醚萜苷可能通过抑制AQP4、MMP-2和MMP-9的表达减轻脑出血后脑水肿的形成。

Objective：To study the influence of total iridoid glycosides in Gardenia jasminoides on the expression of AQP4 and MMP-2/9 in perihematomal brain regions of rats. Methods：Male Sprague-Dawley rats were randomly divided into pseudo-operation group,ICH model group and the treatmengt group of Gardenia jasminoides total iridoid glycosides （30 mg/kg）. ICH models were induced in rats by injecting stereotactic infusion autologous blood into pallidus. The brain tissue specments of 5 rats in every group were respectively gathered at the time of 24h,48h,72h and 120h after operation. The expression of AQP4 protein and gene mRNA and MMP-2/9 in rat after ICH were assessed by inmunohistochemistry and reverse trascriptase polymerase chain reation,at the same time the moisture in brain tissue was measured by dry-wet weighting method. Results：Compared with ICH model group,the expression of AQP4 protein and AQP4 mRNA were decreased obviously,and moisture in brain tissue in Gardenia jasminoides iridoids groups（30 mg/kg） were remarkably declined at every time point. The level of MMP-2/9 in treatment group was lower than that in the model group at 72 to 120 hour after ICH. Conclusion：Gardenia jasminoides total iridoid glycosides groups can reduce the expression of AQP4 and MMP-2/9 in rat after ICH,which may be related with the anti-edema effect folloming ICH.