MicroRNA-205对人皮肤上皮细胞迁移能力的调控

Regulation of microRNA-205 on the migratory ability of normal human epidermal keratinocytes

目的观察microRNA-205(miR-205)在人皮肤上皮细胞中的表达抑制和过量表达对于该类细胞迁移能力的影响。方法使用体外合成的miR-205抑制剂(antagomir-205)处理人皮肤上皮细胞并检测抑制效果,观察其对皮肤上皮细胞迁移的影响。在293TN细胞中包装并收获能过表达成熟miR-205的重组慢病毒颗粒,感染人皮肤上皮细胞并检测过表达效果,进一步观察其对皮肤细胞迁移的影响。结果成功使用miR-205抑制剂实现对人皮肤上皮细胞内源性miR-205的抑制,证实其抑制了皮肤上皮细胞的迁移。成功获得过表达miR-205的重组慢病毒颗粒,证明miR-205可促进皮肤上皮细胞的迁移。结论 miR-205可以促进皮肤细胞的迁移。

Objective To study the influence of miR-205 on normal human epidermal keratinocytes（NHEKs） migration by using synthesized antagomir-205 and recombinant lentivirus expressing miR-205,respectively.Methods Cultured NHEKs were treated with synthesized antagomir-205.Northern blot analysis was conducted to detect whether endogenous level of miR-205 could be reduced by antagomir-205.Wound healing assay was adopted to further measure the cell migratory ability of NHEKs.We also tested its influence on NHEKs by wound healing assay after miR-205 recombinant lentivirus infecting NHEKs.Results The migratary ability of NHEKs was significantly inhibited by antagomir-205,while ectopic expression of miR-205 promoted NHEKs to migrate.Conclusion Ectopic expression of miR-205 promoted the normal human epidermal keratinocytes to migrate.