HIF-1α和HIF-2α在胃癌中的表达及意义

Expression and significance of HIF-1α and HIF-2α in human gastric cancer

目的探讨胃癌中低氧诱导因子HIF-1α和HIF-2α的表达及其临床意义。方法用免疫组化SP法检测组织中HIF-1α、HIF-2α及VEGF的表达;用Western blot法检测组织中HIF-1α、HIF-2α的表达。结果胃癌中HIF-1α、HIF-2α和VEGF的阳性表达率分别为61.5%、36.5%和61.5%,均显著高于正常对照组的11.1%、0和0;HIF-1α表达与VEGF及胃癌的淋巴结转移密切相关。胃癌组织中HIF-1α和HIF-2α蛋白的表达显著高于癌旁正常胃黏膜组织。结论低氧诱导因子的表达可能在胃癌的发生、发展中具有重要作用。

Objective To explore the expression and significance of the hypoxia inducible factor（HIF-1α and HIF-2α） in human gastric cancer.Methods SP immunohistochemical method was used to detect the expression of HIF-1α,HIF-2α and VEGF.Western blot method was used to measure the expression of HIF-1α and HIF-2α in the tissue samples.Results Compared with the normal tissue,gastric cancer had significantly higher expression rate of HIF-1α（61.5% vs 11.1%）,HIF-2α（36.5% vs 0）and VEGF（61.5% vs 0）.The expression of HIF-1α was significantly and correlated with lymph node metastasis and VEGF expression.Expression of HIF-1α and HIF-2α in gastric cancer tissues was significantly higher than that of adjacent non-cancerous gastric mucosa.ConclusionThe expression of HIF-1α and HIF-2 α may play important roles in the development and progression of ganstric cancer.