摘要
目的探讨胃癌中低氧诱导因子HIF-1α和HIF-2α的表达及其临床意义。方法用免疫组化SP法检测组织中HIF-1α、HIF-2α及VEGF的表达;用Western blot法检测组织中HIF-1α、HIF-2α的表达。结果胃癌中HIF-1α、HIF-2α和VEGF的阳性表达率分别为61.5%、36.5%和61.5%,均显著高于正常对照组的11.1%、0和0;HIF-1α表达与VEGF及胃癌的淋巴结转移密切相关。胃癌组织中HIF-1α和HIF-2α蛋白的表达显著高于癌旁正常胃黏膜组织。结论低氧诱导因子的表达可能在胃癌的发生、发展中具有重要作用。
Objective To explore the expression and significance of the hypoxia inducible factor(HIF-1α and HIF-2α) in human gastric cancer.Methods SP immunohistochemical method was used to detect the expression of HIF-1α,HIF-2α and VEGF.Western blot method was used to measure the expression of HIF-1α and HIF-2α in the tissue samples.Results Compared with the normal tissue,gastric cancer had significantly higher expression rate of HIF-1α(61.5% vs 11.1%),HIF-2α(36.5% vs 0)and VEGF(61.5% vs 0).The expression of HIF-1α was significantly and correlated with lymph node metastasis and VEGF expression.Expression of HIF-1α and HIF-2α in gastric cancer tissues was significantly higher than that of adjacent non-cancerous gastric mucosa.ConclusionThe expression of HIF-1α and HIF-2 α may play important roles in the development and progression of ganstric cancer.
出处
《基础医学与临床》
CSCD
北大核心
2010年第6期619-622,共4页
Basic and Clinical Medicine