BMP9促进植入心肌前体干细胞改善心梗大鼠心功能

BMP9 promotes cardiac progenitor cells ameliorate the heart function in rat model of myocardial infarction

目的探讨骨形态形成蛋白9(BMP9)能否促进植入的心肌前体干细胞(CPCs)改善心梗大鼠的心功能。方法用表达绿色荧光蛋白的AdBMP9腺病毒感染CPCs 24 h后,检测CPCs中BMP9的表达量。并将感染AdBMP9腺病毒的CPCs植入心肌梗死大鼠模型中,超声心动图检测左室心功能参数,Masson染色计算心肌梗死面积及观察植入细胞形态学,免疫荧光检测α-MHC及α-actin蛋白的表达。结果 AdBMP9腺病毒感染CPCs 24 h后,BMP9的表达量较其CPCs本身BMP9的表达量有显著增高(P<0.01)。术后4周,植入的感染AdBMP9腺病毒的CPCs及未处理的CPCs均能与周围正常心肌组织融合,且均有心肌特异性结构蛋白α-MHC及α-actin的表达,但较周围正常组织表达差(P<0.05)。同时,植入的心肌前体干细胞可以使心肌梗死面积显著缩小(P<0.05),而BMP9可以加强这一效果(P<0.01)。左室心功能参数结果与梗死面积结果相似。结论 BMP9对移植的心肌前体干细胞改善心梗大鼠心功能有促进作用。

Objective To test whether cardiac progenitor cells（CPCs） induced by bone morphogenetic proteins9（BMP9） can improve the heart function of the rats with myocardial infarction.Methods CPCs were infected by AdBMP9 which express green fluorescent protein.After 24 hours,the expression of BMP9 of infected CPCs was examined by semi-quantitative RT-PCR.Then,AdBMP9 infected CPCs were injected into the infracted heart.The hearts were collected,after postoperative four weeks.The change of CPCs morphology was observed by HE staining and immunofluorescent analysis to investigate the expression of cardiac-specific structural proteins alpha myosin heavy chain（α-MHC） andα-actin of the implanted CPCs.The cardiac infarct size was detected by Masson staining.Heart function was examined by echocardiography,in preoperative and postoperative four weeks.Results After twenty-four hours,the expression of BMP9 of CPCs infected by AdBMP9 improved significantly（P〈0.01）.The result of HE staining and immunofluorescent showed that the grafted-CPCs could fuse with the normal cardiomyocytes and expressed the cardiac-specific proteins α-MHC and α-actin,in the four weeks after transplantation.But the expression of cardiac-specific structural proteins is lower than the normal cardiac tissue（P〈0.05）.During thefour weeks after operation,the implanted CPCs can reduce the sizes of infracted obviously（P〈0.05）and its function can be promoted by BMP9（P〈0.01）.The result of heart function is similar with the Masson staining.Conclusion CPCs induced by BMP9 can improve the heart function of the rats with myocardial infarction.