抑癌基因DPC4蛋白在大肠癌组织中缺失表达及临床意义

The clinical significance of absence of expression of anti-oncogene DPC4 in tissue of colorectal carcinoma

目的观察抑癌基因DPC4蛋白与大肠癌生物学行为的关系,探讨DPC4基因检测能否成为早癌筛检的敏感方法。方法采用免疫组织化学SP法,检测30例正常大肠组织、100例大肠癌中的DPC4基因表达情况。结果 100例大肠癌组织中DPC4阳性率为32%(32/100),正常大肠组织DPC4阳性率100%(30/30),大肠癌的DPC4表达与正常大肠组织DPC4表达的差异有统计学意义(P<0.01)。在大肠癌组织中,DPC4的阳性表达与Dukes’分期、分化程度有关(P<0.05),与年龄、性别无关(P>0.05)。结论检测DPC4基因突变,有助于对肿瘤病因学、发病机制及早期诊断的研究。

Objective To observe the correlation between DPC4 gene and the colorectal carcinoma,discuss the probability of DPC4 gene dectection for early cancer screening. Methods The expression of DPC4 was detected with S-P immuno-histochemical method in samples from 100 cases of colorectal carcinoma,30 cases of normal tissue. Results The positive rates of DPC4 in colorectal carcinoma samples and normal colon samples were 32%（32/100） and 100%（30/30）,respectively. The positive rate of DPC4 was significantly lower in carcinoma tissue （P〈0.01） and showed significant correlation with Duke＇s grading and differentiation. However,no correlation was revealed between DPC expression and age,gender,and carcinoma location （P〈0.05）. Conclusion DPC4 gene mutation shows significant value in study of etiology,pathogenesis and the early diagnosis in carcinoma.