RP-HPLC测定血浆中盐酸利托君浓度

Determination of Ritodrine Hdrochloride in Pasm by RP-HPLC

目的:测定血浆中盐酸利托君的血药浓度。方法:以盐酸苯氧丙酚胺为内标,色谱柱为C18柱,以乙腈-水-磷酸(83:17:0.05,pH2.5)为流动相,流速2ml.min-1,在检测波长200nm进行测定。结果:盐酸利托君血药浓度在5～200ng.ml-1内有良好的线性关系,回归方程为:Y=0.027X+0.011,r2=0.996,RSD=6.8%。结论:所建方法简便、可靠、准确,可用于盐酸利托君血药浓度的测定。

Objective：To determine Ritodrine hydrochloride in the plasm by RP-HPLC.Methods：Isoxsuprine hydrochloride was used as internal standard,C18 column was used with a mixture of acetonitrile-20-hosphoric acid（17：83：0.05,pH2.5）as mobile phase at a flow rate of 2 ml.min^-1 with the detection wavelength at 200 nm.Results：The linear range was 5-200 ng·ml^-1 and the（r^2= 0.996）,The RSD was 6.8%.Conclusion：The method appeared to be simple,reliable and accurate and can be used for the determonatination of Ritodrine hydrochloride in plasm.