青蒿素对人乳腺癌细胞MDA-MB-435辐照后染色体畸变率的影响

Effect of Chromosome Aberration after Irradiation by Artemisinin on Human Breast Cancer Cells MDA-MB-435

目的应用常规染色体分析研究青蒿素对p53功能突变的人乳腺癌细胞MDA-MB-435的放射敏感作用。方法通过MTT法测定不同药物浓度及不同药物作用时间对细胞毒性的影响,常规染色体制片,计数细胞分类项中双着丝粒体(dic)和着丝粒环(r)数量,将经过青蒿素作用的乳腺癌细胞染色体畸变率(dic+r)同单纯照射的细胞染色体畸变率进行统计分析。结果青蒿素对实验细胞MDA-MB-435毒性较小,200μmol/L浓度时作用24h细胞存活率>95%,48h细胞存活率>80%。参考存活曲线确定常规染色体畸变分析实验的药物浓度为200μmol/L,作用时间为24h。将得到的数据进行回归拟合,可以得到药物作用及未经药物作用的剂量效应曲线,比较两组曲线,青蒿素作用组的染色体畸变率明显高于单纯照射细胞(P>0.05)。结论青蒿素能增加辐射对乳腺癌细胞MDA-MB-435的染色体损伤。

Objective Use the chromosome aberration analysis on the p53 mutant human breast cancer cells MDA-MB-435 by using artemisinin,in order to study the radiation sensitizing effect of artemisinin on this cell line.Methods MTT method was used to decide the concentration and the treatment time of artemisinin on the conventional chromosome aberration analysis.Dicentric chromosomes and ring chromosomal aberrations （dic ＋ r） were counted.Results The experimental results showed that：artemisinin is of little toxicity on MDA-MB-435.The drug concentration determined in CB method was 200 μmol/L and the treatment time was 24 h.Conventional chromosome aberration analysis showed that dic＋r chromosome aberration rate of cells treated with artemisinin is significantly higher than that in alone cells only exposed to radiation（P0.05）.Artemisinin can increase chromosome aberrations in the MDA-MB-435 cells after exposure to irradiation,which confirmed that artemisinin had radiation sensitizing effect on human breast cancer cells MDA-MB-435.Conclusion Artemisinin can increase the effects of radiation on breast cancer cells MDA-MB-435 of chromosome damage.