肺特灵Ⅲ对博莱霉素致小鼠肺纤维化的影响

Effects of Fei-te-ling-Ⅲ on Bleomycin-induced Pulmonary Fibrosis in Mice

目的观察肺特灵Ⅲ对博莱霉素致小鼠肺纤维化的干预作用。方法通过气管内注射博莱霉素制作小鼠肺纤维化模型,给予肺特灵Ⅲ灌胃(ig)治疗14d后,进行肺系数、肺组织病理形态及羟脯氨酸(HYP)、总抗氧化能力(T-AOC)、丙二醛(MDA)、谷胱甘肽(GSH)等血清和肺组织中生化指标水平的测定和分析,观察肺特灵Ⅲ对小鼠肺纤维化的影响。结果经肺特灵Ⅲ治疗后,与模型组相比,各组小鼠肺系数和HYP含量显著降低,小鼠血清中T-AOC增强、MDA含量明显降低,肺组织中GSH含量明显增加,肺泡炎和肺纤维化程度明显减轻。上述变化均有统计学意义(皆P<0.05或<0.01)。结论肺特灵Ⅲ对博莱霉素致鼠肺纤维化早期病变有一定的治疗作用,其机制可能与抑制炎症细胞的浸润活化、抗氧化作用及抑制胶原的形成有关。

Objective To study the effects of Fei-te-ling-Ⅲ on bleomycin（BLM） induced pulmonary fibrosis in mice.Methods The pulmonary fibrosis model was estalished by intratracheal injection of Bleomycin.After 14 d treating mice with Fei-te-ling-Ⅲ by intragastric administration,the lung index,the pathomorphology,and the level of hydroxyproline （HYP ）,glutathione （GSH）,malondialdehyde （MDA）,total antioxidant capacity（T-AOC） in lung tissue and blood serum were measured and analysised.The impact of Fei-te-ling-Ⅲ on pulmonary fibrosis in mice was observed.Results After the treatment of Fei-te-ling-Ⅲ,compared with the model group,pulmonary index and the level of HYP were dramatically decreased （P 0.05 or 0.01）.T-AOC in serum was significantly increased,the contents of MDA in serum were significantly decreased,the contents of GSH in lung tissue were significantly increased,Fei-te-ling-Ⅲ could obviously reduced alveolit and pulmonary fibrosis （P 0.05 or 0.01）.Conclusion Fei-te-ling-Ⅲ could have therapeutic effects on the early lesions of bleomycin-induced pulmonary fibrosis in mice.The mechanisms may be related to the inhibition of activation of inflammatory cell infiltration,anti-oxidation and the inhibition of collagen to generate.