摘要
目的观察小建中汤对幽门螺杆菌(Hp)感染小鼠胃黏膜损伤的治疗作用。方法给BABL/c小鼠连续5d灌胃Hp悉尼菌株(SS1Hp)菌液造成Hp感染模型,造模成功后连续4周给小鼠灌胃小建中汤水提组分,给药结束7d后通过胃黏膜直接涂片、快速尿素酶试验及胃黏膜组织细菌培养检查小鼠Hp根除情况,并对胃窦部黏膜进行病理组织学检查。结果小建中汤水提组分高、中、低剂量组的Hp根除率分别为85%、65%、45%,均明显高于模型组(P<0.05或0.01),同时胃窦部黏膜炎症较模型组均有不同程度的改善。结论小建中汤水提组分具有较好的抗Hp感染和抗胃黏膜损伤作用。
Objective To observe therapeutic effect of water extract from Xiao-Jian-Zhong-Tang（XJZT） on injury of gastric mucosa caused by Hp Infection in mice.Methods BALB/c mice were used for model establishment of Hp infection by consecutively ig Hp liquid （109 cufu/mL） for five days.One week after the last infection,mice with Hp serum antibody positive were used as experimental animals in this study.Four weeks after the last bacteria inoculum,the normal controls were not dealt with,and animals in the model group were perfused orally with water,but the other groups were inoculated orally with different dose of XJZT water extract,once a day for four weeks.Seven days after the last treatment,all mice were killed and observed the results of eradiction on Hp and the pathological changes of gastric antrum mucosa.Histology and Hp eradiction were assessed by basic fuchsin staining,Urease test,Hp and HE staining.Results The eradiction rate of Hp in XJZT water extract groups was 85%,65%,45%,it was significant different in groups treated with XJZT at three different doses.The active inflammation of gastrivantrum mucosa was obviously improved and chronic inflammation was slightly improved in mice received drug therapy.Conclusion Water extract from XJZT has an effect on anti-Hp infection and injury of gastric mucosa.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2010年第2期277-279,共3页
Suzhou University Journal of Medical Science
