连云港地区汉族人群15个STR基因座的遗传多态性

Genetic Polymorphism of 15 STR Loci Mutiplexing in Lianyungang Han Population

目的调查连云港地区汉族无关个体的15个STR基因座(D8S1179、D21S11、CSF1PO、D3S1358、D7S820、TH01、D13S317、D2S1338、D18S51、D16S539、TPOX、vWA、D19S433、D5S818、FGA)多态性,研究其在法医学检验中的应用价值。方法用AmpFlSTR IdentifilerTM五色荧光标记系统对新鲜血样进行15个基因座的复合扩增,用ABI3130XL全自动测序仪对扩增产物进行检测,用GeneMapper软件进行基因分型。结果 15个基因座累积个人识别能力值为0.9999999999,累积非父排除率达99.999%,家系调查符合孟德尔遗传规律。结论含有15个STR基因座的五色荧光标记复合扩增系统适合用于个体识别和亲子鉴定。

Objective The genetic studies of 15 short tandem repeats（STRs） loci in AmpFISTR IdentifilerTM multiplex amplification systems was carried out in Chinese Han population in Lianyungang.Methods DNA samples from 500 unrelated individuals were screened.The 15 loci were D8S1179、D21S11、CSF1PO、D3S1358、D7S820、TH01、D13S317、D2S1338、D18S51、D16S539、TPOX、vWA、D19S433、D5S818 and FGA.The PCR products were analyzed and genotyped by ABI 3130XL Sequencer.Results The combined power of discrimination （DP） was 0.999 999 999 9 and the combined paternity of exclusion（PE） was 99.999%.These 15 loci met the Hardy-Weinberg expectations.Conclusion The AmpFISTR IdentifilerTM multiplex amplification systems was very useful in forensic case investigation.