实验性蛛网膜下腔出血后的肿瘤坏死因子变化

The changes of TNF after the experimental SAH in Wistar rat

目的：为探讨蛛网膜下腔出血（SAH）后的神经免疫学变化，采用动物实验方法对实验性SAH后不同时间内的TXB2和TNF－α的变化进行了观察。方法：选用Wistar大鼠做SAH实验模型，经颈骨钻孔往入自家血方法完成实验。对不同时间内的血浆TXB2及TNF－α水平做了放免学测定。结果：TXB2在实验后12h即有显著升高（实验前278．0±54．5mmol／L，12～36h间为2714．6±1248．8～3455．5±1496．9），持续至36h后降至正常。TNF－α在实验后12h亦有显著增高，呈持续性（实验前4．6±1．7mmol／L实验后22．8±8．7mmom／L），但在36h和5d有轻度下降，故增高呈双峰型。结论：实验性SAH后TNP有表达增高改变，其机制多与血管内皮损伤和神经组织受损后的合成增加有关，其作用过程与白细胞和血小板的激活过程相关联。TXB2和TNF早期增高的正相关改变提示TNF增高可能在促凝血和血管痉挛中起一定作用。

Objective The purpose of study was to assess the changes rule of Tumor Necrosis Factor [TNF]. Methods The exiperimental SAH model of Wistar rat were completed by trans-cranal drilling a hole and the reper fusion of selfbloocl, the level of TNF and TXB2 in plasma were determined by radioimmunologic method. Result The time of TXB2 was rised in 12-36h steply ; the increase of TNF was 12h-14d after the exiperiment, the increase changes were double wave after the exiperiment. Conduction.The rise of TNF were a combination effect of cerebral ischemia and the damage of nevous tissure in activiting platlet and WBC after the SAH, the change was directly related with lately vascular spasm. It is significantly to outcome of SAH by reducing production and clearing of TNF.