抗肌球蛋白重链和轻链抗体在扩张型心肌病与冠心病鉴别诊断中的价值

THE VALUE OF ANTIMYOSIN HEAVY CHAIN AND LIGHT CHAIN IN THE DIFFERENTIAL DIAGNOSIS BETWEEN DILATED CARDIOMYOPATHY AND CORONARY HEART DISEASE

目的：旨在探讨抗肌球蛋白重链和轻链抗体，在扩张型心肌病（ＤＣＭ）和冠心病中鉴别诊断的价值。方法；对２１例ＤＣＭ患者，１４例冠心病患者及正常健康组１８例，进行临床免疫学检测，应用ＥＬＩＳＡ法、免疫转印法对检测数值进行分析。结果显示：ＥＬＩＳＡ法ＤＣＭ患者阳性率４３．９％，冠心病组２８．６％，正常对照组均为阴性。免疫转印法２１例的ＤＣＭ患者阳性率４３．９％，冠心组２８．６％，正常对照组均为阴性。免疫转印法２１例的ＤＣＭ患者抗肌球蛋白重链抗体（２００ＫＤ）１０例阳性，阳性率４７．６％，冠心组抗肌球蛋白轻链抗体（２７．５ＫＤ）阳性４例，阳性率２８．６％，ＰＢＳ替代试验均为阴性。

Clinical immunological detection has performed in 21 patients with dilated cardiomyopathy(DCM),14 patients with coronary heart disease(CHD) and 18 healthy people in order to find out the role of antimyosin heavy and light chain in the differential diagnosis between DCM and CHD.ELISA and Immunoblot protein footprint were used for analysis.The results of using ELISA method showed that the positive rate of patients with DCM was 43.9%,of those with CHD was 28.6% and the normal control group was negative.Using protein footprint,the antimyosin heavy chain(200KD) was positive in 10 of 21 patients with DCM,the positive rate was 17.6%.The antimyosin light chain was positive in 4 patients with CHD(27.5%KD),the positive rate was 28.6%.PSB substitution tests were negative for all.It suggested that detection of antimyocardial myosin heavy chain and light chain is useful for the differential diagnosis between DCM and CHD.