期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

YB-1表达对乳腺癌细胞迁移和侵袭能力的影响 被引量：6

Effects of Y-box binding protein-1(YB-1) on invasiveness and migration capability of breast cancer cells
下载PDF
导出
摘要 目的:观察Y-盒结合蛋白-1(YB-1)对肿瘤细胞迁移和侵袭能力的影响。方法:通过脂质体介导的方法将YB-1 shRNA重组载体转染入人类乳腺癌MCF-7细胞,经G418筛选及克隆化培养后获得稳定转染株。运用RT-PCR和蛋白质印迹方法检测MCF-7细胞中YB-1 mRNA和YB-1蛋白的表达水平,运用划痕实验和Transwell小室法检测细胞运动、迁移和侵袭能力的变化,同时运用RT-PCR和蛋白质印迹方法检测细胞中MMP-2的表达水平,并用明胶酶谱法检测MMP-2蛋白的活性。结果:稳定转染YB-1 shRNA的细胞中YB-1的表达明显低于转染随机片段的细胞和未转染组细胞,且细胞的运动、迁移和侵袭能力以及MMP-2的表达及活性也较后两组细胞明显下降。转染随机片段的细胞和未转染组细胞间无明显差异。结论:YB-1 shRNA可以沉默乳腺癌MCF-7细胞中YB-1的表达,并降低细胞的迁移和侵袭能力以及MMP-2的表达及活性。 Objective： To study the effects of Y-box binding protein-1（YB-1） on the invasiveness and migration capability of breast cancer cells.Methods： The recombinant eukaryotic expression plasmid including YB-1 shRNA was introduced into MCF-7 cells by lipofectamine mediation and the cell line with stable expression of YB-1 was obtained by G418 screening and colony culture.The expression levels of YB-1 mRNA and protein were determined by RT-PCR and Western blotting.The mobility,invasiveness and migration capability of cells were evaluated by using wound healing assay and Transwell chamber model in vitro while the expression levels of MMP-2 mRNA and protein were determined by RT-PCR and Western blotting,and the activity of MMP-2 protein was detected by Gelatin Zymography.Results： The expression of YB-1 in the cells transfected with YB-1 shRNA was much less than the cells transfected with random fragment and the control group.The mobility,invasiveness,migration capability and the expression of MMP-2 as well as the activity of MMP-2 protein of the cells transfected with YB-1 shRNA were much lower than the other two groups while the differences between the other two groups were not significant.Conclusion： The interference of YB-1 with YB-1 shRNA in breast cancer MCF-7 cells can suppress the expression of YB-1 and the invasiveness and migration capability of cells,and decrease the expression of MMP-2 and the activity of MMP-2 protein.
作者 杨靖 许文林 李娟 弓晋灵 吴晓君 陈巧云 王法春
机构地区 江苏大学附属人民医院中心实验室
出处 《江苏大学学报（医学版）》 CAS 2010年第3期207-211,共5页 Journal of Jiangsu University：Medicine Edition
关键词 Y-盒结合蛋白-1 MCF-7 SHRNA 迁移和侵袭能力 MMP-2 Y-box binding protein-1 MCF-7 shRNA invasiveness and migration capability MMP-2
分类号 R737.9 [医药卫生—肿瘤]
  • 相关文献

参考文献7

  • 1刘佳,瞿存业,马蕾娜,石文芳,钱其军.YB-1与肿瘤发生及治疗[J].生物化学与生物物理进展,2008,35(7):744-750. 被引量：4
  • 2Giménez-Bonafé P,Fedoruk MN,Whitmore TG,et al.YB-1 is upregulated during prostate cancer tumor progression and increases P-glycoprotein activity[J].Prostate,2004,59(3):337-349.
  • 3Huang J,Tan PH,Li KB,et al.Y-box binding protein,YB-1,as a marker of tumor aggressiveness and response to adjuvant chemotherapy in breast cancer[J].Int J Oncol,2005,26(3):607-613.
  • 4Kotaro S,Kenji S,Toshihiro O,et al.Nuclear expression of the Y-box binding protein,YB-1,as a novel marker of disease progression in non-small cell lung cancer[J].Clin Cancer Res,2001,7(10):3151-3155.
  • 5Matsumoto K,Abiko S,Ariga H.Transcription regulatory complex including YB-1 controls expression of mouse matrix metalloproteinase-2 gene in NIH3T3 cells[J].Biol Pharm Bull,2005,28(8):1500-1504.
  • 6Mertens PR,Harendza S,Pollock AS,et al.Glomerular mesangial cell-specific transactivation of matrix metalloproteinase 2 transcription is mediated by YB-1[J].J Biol Chem,1997,272(36):22905-22912.
  • 7Samuel S,Beifuss KK,Bernstein LR.YB-1 binds to the MMP-13 promoter sequence and represses MMP-13 transactivation via the AP-1 site[J].Biochim Biophys Acta,2007,1769(9/10):525-531.

二级参考文献47

  • 1Grant C E, Deeley R G. Cloning and characterization of chicken YB-1: regulation of expression in the liver. Mol Cell Biol, 1993, 13 (7): 4186-4196
  • 2Tafuri S R, Wolffe A P. Xenopus Y-box transcription factors: molecular cloning, functional analysis and developmental regulation. Proc Natl Acad Sci USA, 1990, 87 (22): 9028-9032
  • 3Li X Y, Mantovani R, He, aft van Huijsduijnen R, et al. Evolutionary variation of the CCAAT-binding transcription factor NF-Y. Nucleic Acids Res, 1992, 20 (5): 1087-1091
  • 4Ozer J, Faber M, Chalkley R, et al. Isolation and characterization of a cDNA clone for the CCAAT transcription factor EFIA reveals a novel structural motif. J Biol Chem, 1990, 265 (36): 22143-22152
  • 5Makino Y, Ohga T, Toh S, et al. Structural and functional analysis of the human Y-box binding protein (YB-1) gane promoter. Nucleic Acids Res, 1996, 24 (10): 1873 - 1878
  • 6Didier D K, Schiffenbauer J, Woulfe S L, et al. Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class Ⅱ Y box. Proc Natl Acad Sci USA, 1988, 85 (19): 7322-7326
  • 7Matsumoto K, Wolffe A P. Gene regulation by Y-box proteins: coupling control of transcription and translation. Trends Cell Biol, 1998, 8(8): 318-323
  • 8Gimanez-Bonafe P, Fedoruk M N, Whitmore T G, et al. YB-1 is upregulated during prostate cancer tumor progression and increases P-glycoprotein activity. Prostate, 2004, 59 (3): 337-349
  • 9Huang J, Tan P H, Li K B, et al. Y-box binding protein, UB-1, as a marker of tumor aggressiveness and response to adjuvant chemotherapy in breast cancer. Int J Oncol, 2005, 26 (3): 607-613
  • 10Gessner C, Woischwill C, Schumacher A, et al. Nuclear YB-1 expression as a negative prognostic marker in nonsmall cell lung cancer. Eur Respir J, 2004, 23 (1): 14-19

共引文献3

同被引文献52

  • 1郭双平,王丽,王文亮,李擒龙,王文勇,张静.抑癌基因PTEN在肝癌组织中的突变及其对肝癌细胞增殖和凋亡的调控作用[J].中华病理学杂志,2006,35(8):467-472. 被引量：25
  • 2Kohno K,Izumi H,Uchiumi T,et al.The pleiotropic functions of theY-box-binding protein,YB-1[J].Biol Essays,2003,25(7):691-698.
  • 3Gu C,Oyama T,Osaki T,et al.Expression of Y box-binding protein 1correlates with DNA topoisomerase II alpha and proliferating cell nucle-ar antigen expression in lung cancer[J].Anticancer Res,2001,21(4A):2357-2362.
  • 4Jurchott K,Bergmann S,Stein U,et al.YB-1 as a cellcycle-regulatedtranscription factor facilitating cyclin A and cyclinB1 gene expression[J].J Biol Chem,2003,278(30):27988-27996.
  • 5Ohga T,Uchiumi T,Makino Y,et al.Direct involvement of the Y-boxbinding protein YB-1 in genotoxic stress-induced activation of the hu-man multidrug resistance 1 gene[J].J Biol Chem,1998,273(11):5997-6000.
  • 6Oda Y,Ohishi Y,Basaki Y.Prognostic implications of the nuclearlo-calization of Y-box-binding protein-1 and CXCR4 expression in ovariancancer their correlation with activated Akt,LRP/MVP andP-glycopro-tein expression[J].Cancer Sci,2007,98(7):1020-1026.
  • 7Gimenez-Bonafe P,Fedoruk M,Whitmore T,et al.YB-1 is upregulat-ed during prostate cancer tumor progression and increases P-Glycopro-tein activity[J].The Prostate,2004,59(3):337-349.
  • 8Dahl E,En-Nia A,Wiesmann F,et al.Nuclear detection of Y-boxprotein-1(YB-1)closely associates with progesterone receptor negativi-ty and is a strong adverse survival factor in human breast cancer[J].BMC Cancer,2009,9:410.
  • 9Gessner C,Woischwill C,Schumacher A,et al.Nuclear YB-1 expres-sion as a negative prognostic marker in nonsmall cell lungcancer[J].Eur Respir,2004,23(1):14-19.
  • 10Kohno K, Izumi H, Uchiumi T. The pleiotropic functions of the Y-box-binding protein YB-1[J]. Bioessays, 2003, 25: 691-698.

引证文献6

二级引证文献9

江苏大学学报（医学版）
2010年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部