Ti2448合金种植体表面不同纳米管径生物活性膜对成骨细胞早期黏附的影响

Effects on Early Adhesion of the Osteoblast to Nanotube-Bio-Active Film on Ti2448 Alloy Implant Surface in Different Diameters

观察Ti2448合金表面不同纳米管径生物活性膜对成骨细胞早期黏附的影响,筛选出能够抵抗细菌在种植体表面黏附和定植的最适管径,为研制开发具有抗菌性能的种植体提供实验基础。采用阳极氧化法,设定不同氧化电压,在Ti2448合金表面生成具有不同纳米管径的生物活性膜。通过MTT实验检测不同管径纳米管表面在不同时间点黏附的MG-63细胞数量差异;通过免疫荧光法观察接种24h后不同管径纳米管表面黏附细胞形态学差异。结果显示,细胞接种3h和24h后,30nm组的OD值显著高于对照组及其他实验组(P<0.05);随着纳米管径的增加,细胞骨架蛋白的铺展范围逐渐减小,细胞由多角形渐变为梭形、椭圆形、圆形。研究结果表明,30nm管径生物活性膜更有利于成骨细胞的早期黏附,可能更能抵抗细菌在钛种植体上的黏附与定植。

In order to provide experimental basis for the researches and development of an implant surface with antibacterial properties,the effects on early adhesion of the osteoblast to nanotube-bio-active film in different diameter on Ti2448 alloy surface were observed,to screen an optimal diameter which could resist the adhesion and colonization of bacteria on the implant surface.Nanotubes in different diameters（NDD）on the surface of Ti2448 alloy were generated adopting anodic oxidation by setting different voltages.The differences of MG-63 cell quantity adhered on the NDD at different time points was detected by MTT assay;The morphological differences of adherent cells on the NDD were observed by immunofluorescence assay 24 h after the inoculation.The results show that 3 h and 24 h after the inoculation the OD value of 30 nm group was significantly higher than that of control group or any other experimental groups（P0.05）;A gradual decreasing spreading area of cytoskeleton actin was observed as the nanotube diameter increased;And the cell configuration changed gradually from polygon to fusiform,ellipse,and round.Therefore,the researches results suggested that nanotubes bio-active film of 30 nm in diameter was more conducive to early osteoblast adhesion and might be more resistant to bacterial adhesion and colonization on titanic implants.