酵母培养物对绒山羊机体抗氧化能力的影响

Effects of Yeast Culture on Antioxidant Ability in Cashmere Goats

本研究旨在探讨酵母培养物(yeast culture,YC)的生物活性及其对绒山羊机体抗氧化能力的影响。选用12只健康的内蒙古白绒山羊半同胞羯羊,按体重配对原则分为4组,分别为A1、A3、B1和B2组。其中A1、A3组饲喂精粗比为3∶7的基础日粮,B1、B2组饲喂精粗比为2∶8的基础日粮。采用自身对照试验方法,对照期饲喂基础日粮,试验期饲喂在基础日粮基础上分别添加不同水平YC的试验日粮。各组YC添加量分别为A1组,2.0%;A3组,3.0%;B1组,2.0%;B2组,2.5%。分别在对照期最后1d、添加YC的第15天、第45天通过颈静脉采血测定绒山羊机体抗氧化指标。结果表明:对照期、添加YC后的第15天和第45天,各组间机体抗氧化能力均没有显著差异(P>0.05)。与对照期相比,添加YC后第15天,A1(P<0.05)、A3(P<0.05)和B1(P<0.01)组血清活性氧的活力显著或极显著降低;添加YC后第15天、第45天,高精料组A1(P<0.01)、A3(P<0.05)的丙二醛(MDA)含量也显著或极显著低于对照期。与对照期相比,试验期各组血清谷胱甘肽过氧化物酶(GSH-Px)的活力提高33.24%～142.52%(P>0.05),显著或极显著提高A3(P<0.01)、B1(P<0.05)组血浆超氧化物歧化酶(SOD)的活力及添加YC后第45天时A1(P<0.01)、A3(P<0.05)、B1(P<0.01)和B2(P<0.01)组的总抗氧化能力(T-AOC)。由此可知,日粮中添加酵母培养物可以提高绒山羊机体的抗氧化能力。

This experiment was conducted to discuss the biologically activity of yeast culture （YC） and its effects on antioxidant ability in cashmere goats. Twelve mature half-sib wethers of Inner Mongolia white cashmere goats were allotted into four groups named A1,A3,B1 and B2. Using a self-contrast designs trial,the goats in A1 and A3 groups were fed a basal diet with concentrate to roughage （C/R） ratio of 3∶7; and the animals in B1 and B2 groups were fed the basal diet with C/R ratio of 2∶8 in control period. After the control period,YC was added at level of 2.0% in A1 and B1 groups,3.0% in A3 group and 2.5% in B2 group in YC supplement period,respectively. Blood samples used to determine the antioxidant ability were taken from the jugular vein on the last day of control period,the 15th day and the 45th day of YC supplement period. The results showed that there was no significant difference on body antioxidant ability among groups in each period （P〈0.05）. Compared with the control period,YC supplementation significantly decreased the activities of serum ROS in A1 （P〈0.05）,A3 （P〈0.05） and B1 （P〈0.01） group on the 15th day of YC supplement period and decreased the concentration of MDA,especially in the animals fed the diet with higher C/R ratio in A1 （P〈0.01）,A3 （P〈0.05） group during YC supplement period. Compared with the control period,YC supplementation increased the activities of serum GSH-Px by 33.24%-142.52% （P〈0.05）,significant enhanced plasma SOD activities in A3 （P〈0.01） and B1 （P〈0.05） group. T-AOC of A1 （P〈0.01）,A3 （P〈0.05）,B1 （P〈0.01） and B2 （P〈0.01） groups were significantly increased at the 45th day of YC supplement period. These results indicated that YC supplementation in diets could increase the antioxidant ability of cashmere goats.