摘要
利用反转录套式聚合酶链反应(RTnestedPCR)技术从北京地区3株鸡传染性支气管炎病毒(IBV)分离株(BJ1,BJ2,BJ3)中扩增出1054bpS1基因高变区。利用限制性内切酶SinⅠ和PstⅠ的酶切位点分析图谱进一步证实了RTnestedPCR的特异性。将3段S1基因分别克隆到pUC19质粒中,获得重组质粒pUCIBVS1BJ1,pUCIBVS1BJ2和pUCIBVS1BJ3。
Using a reverse transcription nested polymerase chain reaction (RT nested PCR), the 1054 bp long S 1 gene hypervariable regions of 3 strains of avian infectious bronchitis viruses (IBV, BJ 1, BJ 2, BJ 3) isolated from Beijing areas were amplified successfully. By means of the map of restriction enzymes SinⅠ and PstⅠ, the specificity of the RT nested PCR products were proved. The recombinant plasmids pUC IBV S 1-BJ 1, pUC IBV S 1-BJ 2 and pUC IBV S 1-BJ 3 were obtained by cloning the three S 1 genes into plasmids pUC 19.
出处
《中国兽医科技》
CSCD
1999年第1期11-14,共4页
Chinese Journal of Veterinary Science and Technology
基金
北京市自然科学基金
关键词
鸡
传染性支气管炎
病毒
S1基因克隆
avian infectious bronchitis virus RT netsed PCR Cloning of the S 1 gene