白细胞介素-23在生殖器疱疹感染中的意义

The significance of interleukin-23 in genital herpes simple virus infection

目的探讨白细胞介素-23(IL-23)在单纯疱疹病毒Ⅱ型(HSV-Ⅱ)致病机制中的作用。方法通过ELISA法检测HSV病毒感染所致复发性生殖器疱疹(RGH)患者外周血IL-23、IL-12、IFN-γ含量,以及外周单个核细胞(PBMC)培养液中IFN-γ浓度,并以不同浓度(0,2 ng/ml,10 ng/ml,50 ng/ml)IL-23进行诱导,同时设立健康对照组进行比较。结果RGH组血清IL-23、IL-12、IFN-γ水平明显低于健康对照组,差异有统计学意义(P<0.01);IL-23剂量依赖性诱导患者PBMC产生IFN-γ,不同浓度间比较,差异均有统计学意义(P<0.01)。结论IL-23在RGH患者体内水平低下,Th1/Th2平衡失调;IL-23能在体外刺激T细胞产生IFN-γ,发挥抗病毒效力。

Objective To evaluate the significance of interleukin-23（IL-23） in genital herpes simple virus infection.Methods The contents of IL-23,IL-12 and IFN-γin peripheral blood in patients with recurrent genital herps（RGH） were detected by ELISA assay,as well as the concentration of IFN-γin monouclear cell culture solution. Besides,the IL-23 was induced at different concentration（0,2 ng/ml,10 ng/ml and 50 ng/ml）,compared with healthy control group.Results The serum levels of IL-23,IL-12,IFN-γlevels were significantly lower in RGH group than healthy control group,and the difference was statistically significant（P0.01）.Furthermore,IL-23 dose-dependently induced PBMC produced IFN-γin RGH,there were significant difference among different concentration （P0.01）.Conclusion It demonstrated that the level of IL-23 was lower in RGH patients,and the Th1/Th2 dysequilibrium. It suggested that the IL-23 may also stimulate in vitro T cells to produce IFN-γ,play an anti-viral effect.