隐丹参酮在猪体内外的代谢转化与抗菌活性

The Metabolism of Cryptotanshinone in Pigs and in Vitro

试验猪经口服及静脉给予抗菌活性成分隐丹参酮后，采用溶剂萃取，ＴＬＣ反复分离、纯化，并用ＵＶ、ＩＲ及１ＨＮＭＲ鉴定。隐丹参酮在猪体内可代谢转化为丹参酮ⅡＡ。体外全血及肝匀浆温孵试验结果表明，隐丹参酮在体外与全血及肝匀浆温孵条件下，仍可被转化为丹参酮ⅡＡ。体外抑菌试验显示，代谢产物丹参酮ⅡＡ仍然具有抗菌活性，但与原药比较存在差异。

The metabolism of cryptotanshinone which isolated from the root of Saliva przewaquskii or S.miltiorrhiza Bunge was studied in pigs and in vitro. The tanshinone ⅡA as a major metabolite was isolated and identified from the urine after intravenous and oral adminstration of cryptotanshinone. The tanshinone ⅡA was also identified in vitro from the incubator consisting of the cryptotanshinone, hipatic homogenate and blood of pigs. The antibacterial test results showed that the metabolite maintained the antibacterial activities to Staphylococcus aureus and Mycobacterium tuberculosis, except for some differences as compared with the parent drug.