内毒素休克山羊NO代谢的变化及山莨菪碱对其影响

Changes of Nitric Oxide Metabolism and Effects of Anisodamine During Endotoxic Shock of Goats

为了探讨一氧化氮（ＮＯ）在内毒素休克病理发生中的作用，将３６头杂交山羊随机分成３组，每组１２只，第Ⅰ组作为对照，第Ⅱ、Ⅲ组颈静脉注射大肠杆菌内毒素２次，间隔２４ｈ，第Ⅲ组于第２次注射内毒素前１０ｍｉｎ静脉注射山莨菪碱（６５４－２），检测血浆、肝、肾、肺组织中的ＮＯ水平和组织中一氧化氮合酶（ＮＯＳ）活性。结果表明，第Ⅱ、Ⅲ组山羊第２次注射内毒素后，血浆ＮＯ水平明显高于第Ⅰ组（Ｐ＜０．０１），其中第Ⅱ组呈持续性升高，７ｈ后第Ⅲ组显著低于第Ⅱ组（Ｐ＜０．０１）；第Ⅱ、Ⅲ组肝、肾、肺组织中的ＮＯ含量和ＮＯＳ活性均明显高于第Ⅰ组（Ｐ＜０．０１），１２ｈ后第Ⅲ组组织中的ＮＯ含量和ＮＯＳ活性显著低于第Ⅱ组（Ｐ＜０．０１）。提示ＮＯ过量为内毒素休克时病理发生中关键的中介机制，预先给予６５４－２则能缓解休克时ＮＯ引起的毒性作用。

For clarifing the role of nitric oxide in pathogenesis of endotoxic shock 36 hybrid goats were randomly divided into three groups of 12 goats each. The goats of group Ⅱand Ⅲ were intravenously injected with endotoxin from E.coli O 111 B 4 dissolved in saline at a dose of 1 800 EU/kg body weight twice for an interval of 24 hours and group Ⅲ goats were injected with anisodamine at a dose 2.5 mg/kg body weight for 10 min prior to second endotoxin treatment. The goats of group Ⅰ were treated with equivolume injection of saline as the normal control. Plasma and tissue nitric oxide (NO) levels and nitric oxide synthase (NOS) activities in liver, lung and kidney were measured during the experiments. The results showed as follows: Plasma NO levels significantly increased in group Ⅱand Ⅲ goats as compared with those in group Ⅰ goats, however, plasma NO levels of group Ⅲ goats were markedly lower than those of group Ⅱ goats 7 h after endotoxin treatment. A significant increase of NO levels and NOS activities in liver, lung and kidney was observed in group Ⅱ and Ⅲ goats 5 h and 12 h after endotoxin treatment in comparison with group Ⅰ. NO levels and NOS activities of liver, lung and kidney in group Ⅲ goats at 12 h after administration of anisodamine were significantly lower than those in group Ⅱ goats treated with endotoxin alone. These studies indicated that nitric oxide production might play a key role in pathogenesis of endotoxic shock, and that anisodamine given antecedently would inhibit nitric oxide induction by endotoxin.