排氟中药方剂的筛选

Selection of Defluorinating TCM Formula

根据中兽医学理论对慢性氟中毒辩证立方，拟定治疗阳虚型的中药方Ⅰ（益智通关散加减）、治疗阴虚型的中药方Ⅱ（健步通关散加减）以及对照方Ⅲ（苁蓉丸），分别用于同期饮含氟水（含氟１５０ｍｇ／Ｌ）的大鼠，剂量均为每日给与生药１ｇ／只，同时设空白对照、中毒对照。通过尿氟、血氟、骨氟、尿羟脯氨酸以及肝、心组织的脂质过氧化物、超氧化物歧化酶、谷胱甘肽过氧化物酶等的检测，确定中毒对照组慢性氟中毒的发生，筛选出有较好排氟效应的中药方剂。结果表明，饮含氟水（１５０ｍｇ／Ｌ）的中毒对照大鼠，９０ｄ时出现慢性氟中毒症状，而应用各中药方的大鼠均未出现慢性氟中毒的临床症状。中药方效果对比，中药方Ⅰ比中药方Ⅱ能明显地促进氟从尿排出，降低骨氟蓄积，减少尿中游离羟脯氨酸含量。在抑制脂质过氧化物含量升高和提高超氧化物歧化酶活性方面，中药方Ⅰ明显优于中药方Ⅱ和对照方Ⅲ。

According to the principle of traditional Chinese veterinary medicine, a chronic fluorosis (CF) model of rats was established and three different prescriptions-Yang insufficiency curing recipe Ⅰ, Ying deficiency curing recipe Ⅱ and the control recipe Ⅲ were chosed. The fluorine contents in urine, serum and bone, and the hydroxyproline (HYP) in urine were measured. The effect of the three recipes on the lipid peroxide level, superoxide dismutase (SOD) and glutathione peroxidase (GSH Px) activities of heart and liver were also described. The results showed: ①The Wistar rat drunk water only containing fluorine 150 mg/L occurred the CF symptoms at the end of third month. ②The recipe Ⅰ was more effective than recipe Ⅱ in enhancing the ural excretion of fluorine, lowering the fluorine deposition of bone and the HYP level in urine, and in antagonizing lipid peroxidation and increasing the SOD activity.