GC/MS法测定尿中的巴比妥酸盐(英文)

Determination of Barbiturates in urine by GC/MS

目的建立高特异性﹑高敏感性可分离及鉴别人体尿液中巴比妥类药物的程序方法。方法①用Toxi-tube提取人体尿液中的N,N-二甲基甲酰胺二甲基缩醛衍生物(DMF-DMA)。②运用GC/MS﹑SIM系统及药品定量软件系对人体尿液中巴比妥类药物:布他比妥,异戊巴比妥,戊巴比妥,司巴比妥,苯巴比妥进行定性定量分析。结果①GC/MS对人体尿液中司巴比妥最低检出浓度为30 ng/mL,对布他比妥﹑异戊巴比妥﹑戊巴比妥﹑苯巴比妥最低检出浓度为200 ng/ml。②在实验的运行操作中该方法 /程序显示很高的精确度和准确度。结论此检测方法选择性好﹑检测简便﹑灵敏﹑定性定量结果准确,可用于巴比妥类药物中毒及巴比妥类药物滥用的临床检验诊断。

Objective To introduce a highly selective and sensitive procedure for isolating and identifying barbiturates in human urin established by University of kentucky,USA.Methods ①An aliquot of urine is extracted with a Toxi-tube and derivatized with N,N-dimethylformamide dimethyl acetal（DMF-DMA）.② The sample is analyzed by GC/MS and selected ion monitoring mode（SIM） was used for identification Drug Quant software was used for quantification.for the following barbiturates： butalbital,amobarbital,pentobarbital,secobarbital and phenobarbital in human urin.Results ①The cutoff level for GC/MS is 100 ng/ml for amobarbital,butalbital,pentobarbital,phenobarbital and 30 ng/mL for secobarbital.②The method/procedure also showed high between run and within run precision and accuracy.Conlusions This method/procedure is more selective,more sensitive,simpler,more accurate,and can be applied to clinical diagnosis of barbiturates poisoning and barbiturates abuse.