摘要
目的探讨特发性血小板减少性紫癜(ITP)患者外周血树突状细胞(DC)亚群的变化及其临床意义。方法用流式细胞技术检测29例初治ITP患者及15例健康人外周血CD11c+树突状细胞(DC1)、CD123+树突状细胞(DC2)亚群的数量。以上29例患者按1mg/kg.d的泼尼松常规治疗两周,分析ITP患者外周血树突状细胞亚群数量变化与短期疗效的相关性。结果与正常对照组比较,29例ITP患者外周血CD123+DC比例及绝对数有明显降低(P<0.05,)而CD11c+DC比例和绝对数无显著差异(P>0.05)。29例ITP患者中经泼尼松常规治疗两周后有效的患者有16例,无效的有13例,将这两组治疗前的外周血DC亚群与健康对照组比较发现,有效组的CD123+DC有明显下降(P<0.05),CD11c+DC无明显差异(P>0.05)。而无效组CD123+DC及CD11c+DC均无明显差异(P>0.05)。结论树突状细胞亚群的异常可能与部分ITP的发病有关。DC2下降的ITP患者经糖皮质激素治疗后的短期疗效可能更好。
Objective To investigate the quantity of circulating dendritic cells（DC） in patient with idiopathic thrombocytopenic purpura（ITP） and the clinical significance.Methods The flow cytometer was used to analyze the number of CD11c^＋DC（DC1）,CD123^＋DC（DC2） in 29 cases of patients with ITP and 15 cases of healthy controls.Results The ratio and absolute numbers of circulating CD123^＋DC of patients were decreased in ITP patients（P〈0.05）,the ratio and absolute numbers of circulating CD11＋DC were no significantly different between patients and healthy controls（P〉0.05）.After two weeks treatment by dehydrocortisonei,n the 29 cases of patients with ITP,16 cases of them were efficacious,and 13 of them were inefficacious,comparing the ratio and absolute numbers of circulating DCs subsets in the two groups with that of the healthy controls,we found that the ratio and absolute numbers of circulating CD123^＋DC of efficacious patients were lower than healthy controls（P〈0.05）,the ratio and absolute numbers of circulating CD11c^＋DC were no different（P〉0.05）.However,the ratio and absolute numbers of circulating CD11c^＋DC and CD123^＋DC witch of inefficacious patients were no difference than healthy controls（P〉0.05）.Conclusions DCs may play a role in the pathogenesis of ITP.Those patients who’s ratio and absolute numbers of circulating CD123 ＋DC decreas would be get a better efficacy than others,when they accept the treatment of dehydrocortisone.
出处
《遵义医学院学报》
2010年第1期26-28,31,共4页
Journal of Zunyi Medical University
基金
遵义医学院基金项目(200625号
200609号)
