摘要
应用正交实验研究了生长调节物质病毒唑（ｒｉｂａｖｉｒｉｎ）、茉莉酸甲酯（ｊａｓｍｏｎｉｃａｃｉｄｍｅｔｈｙｌｅｓｔｅｒ）、油菜素内酯（ｂｒａｓｉｎｏｌｉｄｅ）、腐殖酸钠（ｓｏｄｉｕｍｈｕｍａｔｅ）对组织培养暗紫贝母（ＦｒｉｔｉｌａｒｉａｕｎｉｂｒａｃｔｅａｔａＨｉｓａｏｅｔＫ．Ｃ．Ｈｓｉａ）小鳞茎生长的影响，发现病毒唑有显著活性。进一步对不同浓度病毒唑的效果进行优化试验，结果表明：病毒唑１０ｍｇ／Ｌ是提高小鳞茎生长率的最适浓度。
Growth regulators including ribavirin, jasmonic acid methyl ester, brassinolide and sodium humate were added into the media with a view to determine their effect on cultured bulblets of Fritillaria unibracteata Hisao et K. C. Hsia. Results from the orthogonal experiments indicated that ribavirin had significant promotive activity. Further optimizing experiment on the effect of different concentration of ribavirin showed that 10 mg/L ribavirin was the optimized concentration in promoting growth of induced bulblets.
出处
《植物资源与环境》
CSCD
1999年第1期52-55,共4页
Journal of Plant Resources and Environment
基金
国家中医药局重点课题
关键词
生长调节物质
暗紫贝母
组织培养
病毒唑
贝母
growth regulators
Fritillaria unibracteata Hisao et K. C. Hsia
tissue culture
ribavirin
