丙酮酸对肺炎克雷伯氏菌微氧发酵甘油产1,3-丙二醇的影响

Effect of pyruvate on microaerobic fermentation of 1,3-propanediol from glycerol by Klebsiella pnenmoniae

微氧条件下,考察肺炎克雷伯氏菌发酵生产1,3-丙二醇过程中柠檬酸和丙酮酸对发酵过程的影响。摇瓶实验结果表明:添加柠檬酸能抑制菌体生长和1,3-丙二醇合成;丙酮酸对菌体生长和1,3-丙二醇合成有一定的促进作用。5 L发酵罐批式发酵表明:补料培养基中加入8 g/L丙酮酸,1,3-丙二醇的产量提高了约10.8%,转化率提高了约4.4%,比生长速率提高了约10.8%。上述结果初步表明,强化能量的产生能够有效促进1,3-丙二醇的合成,可以利用分子生物学手段强化丙酮酸的产生以促进1,3-丙二醇的合成。

Under microaerobic conditions,effects of citrate and pyruvate on 1,3-propanediol（1,3-PD） fermentation by Klebsiella pnenmoniae were studied.The experiments were carried out in shake flask.The results showed that the enhancement of cell growth and 1,3-PD synthesis was found by adding pyruvate,while the inhibition of those was found with the addition of citrate.Fed-batch fermentation was then studied in a 5 L fermentator by the addition of pyruvate into feeding.The final concentration of 1,3-PD increased about 10.8% with the convert ratio and the specific growth rate of 4.4% and 10.8%.Results showed that enhancement of 1,3-PD synthesis was improved by adding ATP.