抗传染性法氏囊病病毒单克隆抗体的制备及其生物学特性鉴定

Development and Biological Properties of Monoclonal Antibody Against Infectious Bursal Disease Virus

应用杂交瘤技术获得了１３株抗传染性法氏囊病病毒（ＩｎｆｅｃｔｉｏｕｓＢｕｒｓａｌＤｉｓｅａｓｅＶｉｒｕｓ，ＩＢＤＶ）的单克隆抗体（ＭｏｎｏｃｌｏｎａｌＡｎｔｉｂｏｄｙ，ＭｃＡｂ），其中有９株ＭｃＡｂｓ为ＩｇＧ类，４株为ＩｇＭ类，这些ＭｃＡｂｓ与新城疫病毒（ＮｅｗｃａｓｔｌｅＤｉｓｅａｓｅＶｉｒｕｓ，ＮＤＶ）、传染性支气管炎病毒（ＩｎｆｅｃｔｉｏｕｓＢｒｏｎｃｈｉｔｉｓＶｉｒｕｓ，ＩＢＶ）和马立克氏病病毒（Ｍａｒｅｋ’ｓＤｉｓｅａｓｅＶｉｒｕｓ，ＭＤＶ）均无交叉反应，病毒中和试验表明，有５株ＭｃＡｂｓ具有中和活性，Ｗｅｓｔｅｒｎ－ｂｉｏｔｉｎｇ试验表明，这５株有中和活性的ＭｃＡｂｓ识别的抗原位在ＶＰ２上。

Thirteen monclonal antibodies(McAbs) were produced against infectious bursal disease virus(IBDV)using hybrodoma technique.Nine of them are IgG classes and four are IgM Classes.The have no cross reaction with NDV、IBV and MDV.The result of virus neutralization test showed that five McAbs had nutralizing activity.Western-blotting indicated that the epitopes recognized by neutrality McAbs were all present on VP 2 of IBDV.