复方丹参滴丸对急性心肌梗死大鼠基因表达谱的影响

Studies on the Effect of Compound Danshen Dripping Pills on gene expression of acute myocardial infarction model in rats

目的:利用基因芯片技术对复方丹参滴丸对心肌梗死大鼠基因表达的影响进行评价和研究。方法:使用冠脉结扎法造成大鼠心肌缺血模型,并提取给药组和模型组大鼠心肌缺血区组织的总RNA,用大鼠全基因组芯片分析其基因组表达谱变化。结果:基因芯片结果显示,与模型组相比,给药组表达量发生变化的基因(Ratio>2或<0.5)有49条,其中表达下调的有39条,主要有Ppp3r1、Rhoa和Nppa,表达上调的有10条,包括H2-T24、RT1-CE15、Ptprb和Hcst等。结论:复方丹参滴丸可通过调节Wnt信号通路等多个基因和信号途径来减小心肌梗死模型大鼠心脏缺血区的心肌损伤。

Objective： To investigate the role of traditional Chinese medicine Compound Danshen Dripping Pills in the regulation of immune-related genes expression in acute myocardial infarction. Methods： Myocardial infarction was induced by ligation of the left anterior descending artery. Isolated the total RNA from ischemia tissue of Compound Danshen Dripping Pills group and model group, and then screened the differently expressed genes with the gene chip technique. Results： There were 49 regulated genes by Compound Danshen Dripping Pills compared with the model, 39 genes were down-regulated, including Ppp3r1, Rhoa and Nppa; 10 genes were up-regulated such as H2-T24, RT1-CE15, Ptprb and Hcst. Conclusions： Some genes and signaling pathway such as Wnt signal pathway could be regulated to reduce the myocardial damage of ischemia tissue by Compound Danshen Dripping Pills.