治咳川贝枇杷滴丸镇咳平喘作用研究

Antitussive and anti-asthmatic effect of Zike Chuanbei Pipa Diwan in guinea pigs

目的:研究治咳川贝枇杷滴丸对豚鼠的镇咳、平喘作用。方法:采用豚鼠离体气管平滑肌收缩实验和组胺引喘的活体动物实验对治咳川贝枇杷滴丸的平喘作用进行评价。在离体实验中测定药物对乙酰胆碱引起的气管平滑肌收缩的收缩曲线,计算收缩百分率;在整体动物实验中测定给药后豚鼠的延喘时间用来表征药物平喘作用的强弱。同时采用枸橼酸诱发豚鼠咳嗽模型,通过记录引咳后5min内的咳嗽次数,观察治咳川贝枇杷滴丸的镇咳作用。结果:治咳川贝枇杷滴丸能有效减少豚鼠的咳嗽次数,显著延长咳嗽潜伏期;对豚鼠离体气管收缩的抑制效果较好;对组胺致喘豚鼠的哮喘潜伏期有明显的延长作用。结论:治咳川贝枇杷滴丸具有良好的镇咳、平喘药理活性,为药物的临床应用和进一步开发提供了依据。

Objective：To study antitussive and anti-asthmatic effect of Zhike Chuanbei Pipa Diwan（ZCPD） in guinea pigs.Methods：Guinea pig models of isolated trachea in vitro and hitamine-induced asthma in vivo were used to investigate the anti-asthmatic effect of ZCPD.In vitro experiment,concentration-response curves of contractile isolated trachea induced by acetylcholine（Ach） were obtained and the concentration producing 50% of the maximal response was determined.In vivo experiment,the latent period of animals after administration was uesd to demonstrate the anti-asthmatic effect.Meanwhile,the aminal cough model induced by citric acid was developed to evaluate the antitussive effect.Qualified guinea pigs were divided into five groups randomly：high,medium,low concentration ZCPD groups,pentoxyverine positive control group,and control group.The number of cough during three minutes was recorded after the cough was induced.Results：ZCPD decreased the frequency of cough and prolonged the latency of induced cough.Morever,it significantly inhibited Ach-induced trachea contractions and prolonged the latent period of asthma by hitamine in guinea pigs.Conclusion：ZCPD has remarkable antitussive and anti-asthmatic effects,which provides the evidence for clinical application and further development.