RP-HPLC法测定固公果中蔷薇酸和委陵菜酸

Determination of euscaphic acid and tormertic acid in roots of Rosa odorata var.gigantean by RP-HPLC

目的:利用RP-HPLC方法测定彝族药物固公果中蔷薇酸、委陵菜酸的量。方法:采用UltimatexB-C18(美国Welch公司,150nm×4.6mm,5μm)色谱柱,以甲醇-1.5%磷酸水溶液(33︰67)为流动相,体积流量1.0mL/min,检测波长210nm。结果:固公果中蔷薇酸与委陵菜酸能得到很好的分离。测定线性范围分别为:蔷薇酸77.4～774.3μg/mL,r=0.9999;委陵菜酸54.7～546.9μg/mL,r=0.9999。蔷薇酸平均回收率为102.5%(n=9),委陵菜酸平均回收率为103.7%(n=9)。结论:该法便捷、灵敏、准确,重复性好,可以控制该药材的质量。

Objective：To establish a RP-HPLC method for determination of euscaphic acid and tormertic acid in the root of Rosa odorata var.gigantea.Methods：A Ultimatex B C18 column（150 nm × 4.6 mm,5 μm） and a mobile phase consisted of methanol-0.15% phosphoric acid（33︰67） were used for analysis.The flow rate was 1.0 mL/min and the UV detector wavelength was 210 nm.Results：Euscaphic acid was well separated from tormertic acid and other compounds in R.odorata var.gigantea.The calibration curves of euscaphic acid and tormertic acid had a good linearity（r=0.999 9） in the range of 0.077 4﹣0.774 3 mg/mL and 0.054 7﹣0.546 9 mg/mL,respectively.The average recovery of euscaphic acid and tormertic acid were 102.5%（n=9） and 103.7%（n=9）.Conclusion：This method is simple,accurate,and reproducible,which is suitable for the quality control of euscaphic acid and tormertic acid in the roots of.R.odorata var.gigantea.