超声波强化溶剂法提取勿忘我花色素的工艺研究

Study on Solvent Extraction Technique of Limonium sinuatum Pigment Enhanced by Ultrasonic Wave

研究了超声波强化溶剂法提取蓝紫色勿忘我花色素的工艺,包括超声提取和浸泡提取结果的比较、提取条件对提取色素的影响,并用正交试验法探索最佳工艺条件。实验结果表明超声提取比浸泡提取效率高,最佳提取工艺条件：用60%乙醇（pH值2）为溶剂,原料与溶剂比例1∶80-1∶100（g∶mL）,在40℃下超声提取30 min,经过滤、真空浓缩、石油醚洗涤、冷冻干燥得到紫红色固体色素。色素平均得率15%,色价（540 nm）为36.55。用纸色谱分离色素,得到2个斑点,分别为红色和黄色。对这2个组分分别做了紫外可见吸收光谱和显色反应,认为是花青素和黄酮类物质。

Extraction technique of the pigment from violet flowers of Limonium sinuatum were studied,including comparison between ultrasonic extraction and soak extraction,effect of extraction conditions on pigment,and the optium conditions were found by using orthogonal test.Experiments indicated that the efficiency of ultrasonic extraction was higher than soak extraction.The optimum conditions were as follows： ratio of petals to solvent 1∶80-1∶100（g∶mL）,solvent 60% ethanol（pH value 2） and ultrasonic extraction for 30 min at 40℃.Then red solid pigment was obtained through filtration,vacuum concentration,extracting by petroleum ether and lyophilization.The average yield of pigment was 15%,and color scale was E1%1cm（540nm）=36.55.Two spots,red and yellow were acquired using paper chromatography to separate pigments.Two components were determined by UV-Vis absorption spectra and the color reaction separately.The result showed that the pigment were mixture of anthocyanins and flavones.