醒神解毒方预处理减轻全脑缺血再灌注大鼠海马神经元损伤

An Experimental Study on Xingshen Jiedu Decoction Preconditioning Effect on Cerebral Ischemic-Refusion Injury in Gerbils

目的:观察比较醒神解毒方预处理与缺血预处理减轻全脑缺血再灌注大鼠海马神经元损伤的作用。方法:实验选用24只雄性SD大鼠,随机将24只大鼠分为假手术组、缺血预处理组、药物处理组、脑缺血再灌注组,采用热凝椎动脉,钳夹双侧颈总动脉建立全脑缺血模型,缺血预处理组预缺血3min,3天后给予缺血10min,再灌注24h后处死。假手术组暴露双侧颈总动脉不夹闭。缺血再灌注组,夹闭双侧大脑颈总动脉10min,再灌注24h后处死。药物预处理组,灌胃2周后,做缺血再灌注处理,运用HE染色光镜下观察存活海马神经元细胞数,电镜下观察海马神经元超微形态的改变。结果:药物预处理存活神经元与缺血预处理存活神经元比较,P>0.05,两者与缺血再灌注比较,P<0.01。假手术组可见海马神经元细胞形态正常,脑缺血预处理组和药物预处理组海马CA1区神经元损伤明显减轻,只有少量细胞有轻度水肿,未见坏死。而脑缺血再灌注组神经元变性严重。结论:药物预处理对随后的脑梗死有明显的保护作用,能诱导缺血耐受的产生。

Objective：To investigate the preconditioning effects of Xingshen Jiedu Decoction and the effect of ischemic preconditioning on the changes of structure.Method ：Twenty-four male SD rats（weight 180～220g）were randomly divided into 4 groups ：Sham operation group（n=6）;the skin was sutured after common carotid artery（CCA）was exposed ;ischemic group（n=6） ;ischemic preconditioning group（n=6） ;Xingshen Jiedu Decoction pretreatment group（n=6）.Global cerebral ischemia and reperfusion model were established with Pulsinelli＇s method.Result ：Compared with ischemic group,the number of survival neuron of hippocampal CAl region was increased significantly in Xingshen Jiedu Decoction pretreatment group and ischemic precondioning group（P0.05）.Compared Xingshen Jiedu Decoction pretreatment group with ischemic precondioning group,the number of survival neuron had no distinct significance in the hippecampal CA1 region（P0.05）.Conclusion ：Ischemic preconditioning can obviously increase brain tissue tolerance of the brain to secondary severe ischemia.