肽文库富集荷斯坦奶牛血浆和血清中低丰度蛋白的效果研究

Protein profiling of enrichment of low-abundance proteins in bovine plasma and serum using hexapeptide library

【目的】获取牛血浆和血清蛋白质组中低丰度蛋白的表达信息。【方法】采用肽文库试剂盒ProteoMiner富集荷斯坦奶牛的血浆和血清,运用二维凝胶电泳结合液相色谱串联质谱方法,分析富集前后血浆和血清中蛋白的变化。【结果】牛血浆和血清经ProteoMiner试剂盒富集,白蛋白含量显著下调(血浆和血清的差异显著水平分别为P=0.02和P=0.01),载脂蛋白E显著增加(血浆和血清的差异显著水平分别为P=0.02和P=0.01)。【结论】该法在降低牛血浆和血清中高丰度蛋白的同时有效富集了低丰度蛋白,有利于血浆和血清中低丰度蛋白质组的研究。

【Objective】 The objective of this study was to improve the expression of low-abundance proteins in bovine plasma and serum.【Method】 Low-abundance proteins in bovine plasma and serum were enriched by ProteoMiner kit.Plasma and serum proteins of the dairy cows were separated and identified using two-dimensional electrophoresis（2-DE） combinated with HPLC tandem ion trap spectrometry.【Result】 The results showed that,albumin abundance was down-regulation（plasma P=0.02 and serum P=0.01,respectively）,at the same time,the abundance of other protein such as apolipoprotein E was increased（plasma P=0.02 and serum P=0.01,respectively）.【Conclusion】 The findings may provide valuable information for the research of low abundance proteome in plasma and serum.