Fas-L在宫颈癌中的表达及其临床意义

Lmmunohistochemical Analysis of Fas-L Protein In Carcinogenesis

目的研究凋亡配体Fas-L(Fas ligand)在宫颈癌中的表达及其与宫颈肿瘤发生发展之间存在的关联。方法采用免疫组织化学法检测12例正常宫颈上皮组织、18例宫颈上皮内瘤变(CIN)组织和40例宫颈癌组织中Fas-L蛋白的表达。结果宫颈癌组织中,Fas-L染色多见于整个病变全层;在正常宫颈、CIN和宫颈癌组织中Fas-L的阳性表达呈明显递增趋势(P<0.01);与宫颈癌组织学分级呈正相关关系(Rs=1,P<0.05)。结论 Fas-L可能在宫颈癌的发病过程中起到一定的作用。

Objective This study aims to examine if change in death ligand Fas-L expression during different stages of cervical carcinogenesis are related to development of cervical carcinogenesis.Methods Immunohistochemical method was used to detect the expressions of Fas-L in 40 samples of cervical carcinoma,and l8 samples of cervical intraepithelial neoplasia（CIN）,together with 12 samples of normal cervical tissues.The level of the expression was analyzed combined with clinicopathological features of age and differentiation degree,histology type and lymph node metastasis.Results In normal cervix and CIN samples,Fas-L staining was mainly observed in the basal/parabasal layer,wherease Fas-L expression was more frequently observed in cervical.The rate of positive expression of Fas-L was 33.33%,55.56% and 80% in normal cervical tissues,cervical intraepithelial neoplasia（CIN）and cervical carcinoma,and the tendency of increase was obvious（P〈0.01）;there was a significant difference between Fas-L expressions and histologic grades,the higher expression rate of Fas-L expression were found in those poorer differentiated tumors（P〈0.01）;FasL correlated with lymphnode metastasis;there was no significant difference between Fas-L expression and age of patient as well as clinic type.Conclusion The deregulation of Fas-L in the CIN-cercical cancer sequence suggest a possible functional role of these death ligands during cervical carcinogenesis.The frequent expression of them presents these death ligands as promiding targets for innovative therapy modalities in cervical cancer.