Notch信号通路在体外培养的鹿茸干细胞中的表达

Notch Signaling Pathway Expressed in Cultured Antler Stem Cells Cultured in Sika deer

Notch信号通路是一条进化上十分保守的信号转导系统,在调节干细胞增殖、分化和凋亡方面起到重要作用。研究表明,鹿生茸区骨膜和角柄骨膜分别含有鹿茸发生和再生的干细胞。应用RT-PCR的方法对离体培养生茸区骨膜和角柄骨膜细胞进行检测,得出Notch信号通路各信号因子在2种细胞中的表达情况。结果:Notch-1、Notch-2、Notch-4、Dll-4J、agged-1J、agged-2、Hes-1等信号因子在这2种干细胞中均有不同程度的表达,说明Notch信号通路可能参与了他们的增殖、分化的调控。

Notch signaling pathway is an evolutionarily conserved system and plays an important role in stem cell biology including proliferation,differentiation and apoptosis.To isolate,culture and purify deer antler stem cells including AP and PP cells,expression pattern of notch signaling molecules in the cultured AP cells and PP cells were examined using RT-PCR.Data obtained from RT-PCR showed that：the mRNA of Notch-1,Notch-2,Notch-4,Dll-4,Jagged-1,Jagged-2 and Hes-1 had different levels of expression in AP and PP cells,which indicates that notch signaling pathway may be involved in the maintenance of the AP and PPcells.