二个毛白杨新杂种组培再生体系的建立研究

Research of Establishment of Tissue Culture Regeneration System About Two New Hybrid Poplars

以毛白杨×响叶杨,毛白杨×84K杨2个新杂种的无菌苗嫩茎为外植体,进行组织培养。研究其植株再生能力,筛选出较为理想的芽增殖和生根培养基,建立起有效的茎段增殖快繁途径。结果表明,毛白杨×响叶杨的最佳增殖培养基是MS+6-BA 0.5 mg/L+NAA 0.01 mg/L,茎芽诱导率为93.3%,平均分裂芽数为6.38个。毛白杨×84K杨的最佳增殖培养基是MS+6-BA 1.00 mg/L+NAA 0.01 mg/L,茎芽诱导率为90.0%,平均分裂芽数6.00个。毛白杨×响叶杨的最佳生根培养基是MS+NAA 0.15 mg/L,生根率达到86.7%,毛白杨×84K的最佳生根培养基是MS+NAA 0.15 mg/L,生根率96.7%。

Populus tomentosa × Populus adenopoda and Populus tomentosa × 84K poplar were two new hybrids. Sterile shoots of them were used in this tissue culture study as explants. Research on the potential of plantlet regeneration was compared in order to filter out a more satisfactory bud proliferation and rooting medium, establish effective ways to the stern-proliferation of fast propagation. The results showed that the best multiplication medium for Populus tornentosa X Populus adenopoda was MS ＋-6-BA 0. 5 mg/L ＋ NAA 0. 01 mg/L and the shoots proliferation rate was 93. 3%, the average number of proliferating stem axillary buds was 6.38. And for the Populus tomentosa × 84K poplar was MS ＋6- BA 1.0 mg/L ＋ NAA 0. 01 mg/L and the shoots proliferation rate was 90. 0%, the average number of proliferating stem axillary buds was 6.00. The best rooting medium for Populus tornentosa× Populus adenopoda was MS ＋ NAA 0. 15 mg/L, rooting rate reached 86. 7%,and the Populus tomentosa × 84K was MS ＋ NAA 0. 15 mg/L, rooting rate was 96. 7 %.