鸡胚致死率作为检验鸡大肠埃希菌致病力指标的探讨

Embryo Lethality Assay for Determining Virulence of Avian Escherichia coli

为探讨鸡胚致死率在检验鸡大肠埃希菌致病力方面的应用潜力，试验采用SPF级12日龄鸡胚和1日龄公雏鸡分别接种鸡大肠埃希菌菌株，检测20株鸡大肠埃希菌菌株对12日龄鸡胚和1日龄公雏鸡的致死率的相关性。结果显示，8株来源于患败血症鸡只的菌株致病力比较高，鸡胚致死率达到40％～70％；而来源于粪便的12株菌株中，10株菌株的致病力较低，其鸡胚致死率在0％～20％之间；雏鸡致死率与鸡胚致死率是相关的。表明鸡胚致死试验可反映出大肠埃希菌的毒力强弱，即鸡胚致死率可作为检验鸡大肠埃希菌致病力强弱的一种方法。

In order to determine the potential of ELA（embryo lethality assay） for determining virulence of avian E. coli, an embryo lethality assay of 20 isolates was linked with a chicken ethality assay to analyze their association. In this study, 8 isolates from colisepticemia were higher virulent, with 40 %-70% embryo lethality. 10 of 12 from chicken feces were less virulent, with 0%-20% embryo lethality. And the embryo lethality was associated with chicken lethality. This study suggested that ELA may be correlated with virulence of avian E. coli, and may be a method for determining virulence of avian E. coll.