丝瓜中维生素C含量的测定

Determination of Vitamin C in Luffa

为建立丝瓜中维生素C含量直接测定的新方法,以10%盐酸溶液为溶剂,采用紫外分光光度法测定其含量。结果表明,维生素C在0～10 mg/L质量浓度范围内与吸光度呈良好的线性关系,回归方程为:y=0.047 46ρ+0.001 09,r=0.999 7。检出限为0.023μg/mL,加标回收率在90.6%～99.0%之间。该法操作简单,结果准确,应用于维生素C含量的测定,结果准确,令人满意,可作为控制维生素C质量的方法。

To establish a new ways of direct determination of vitamin C content of Loofah,hydrochloric acid concentration of 10% as the solvent,the concentration of vitamin C in Loofah by UV spectrophotometry.The results vitamin C in the 0～10 mg/L concentration range of absorbance within the linear regression equation is： y=0.047 46 ρ ＋0.001 09,r = 0.999 7.The detection limit is 0.023 μg/mL,and the recovery in between 90.6%～99.0%.It concludes this method is simpler.The results are more accurate.Apply to the determination of vitamin C.The results are satisfactory,accurate and can be used as a way of control of vitamin C quality.