花生四烯酸和亚油酸对肝星状细胞增殖的影响

Effect of arachidonic acid and linoleic acid on proliferation of rat hepatic stellate cells *

目的探讨花生四烯酸和亚油酸对大鼠肝星状细胞（ＨＳＣ）增殖的影响．方法用链霉蛋白酶和胶原酶原位灌流，ｎｙｃｏｄｅｎｚ密度梯度离心分离大鼠肝星状细胞，并以ＭＴＴ比色法观察花生四烯酸和亚油酸对ＨＳＣ增殖效应．结果花生四烯酸和亚油酸可影响ＨＳＣ的增殖．花生四烯酸２５ｍｇ／Ｌ对ＨＳＣ有增殖作用（Ｐ＜００１），而５０，１００ｍｇ／Ｌ对ＨＳＣ增殖有抑制作用（Ｐ＜００１），倒置显微镜观察可见ＨＳＣ增殖非常缓慢，ＨＳＣ细胞轮廓非常明显，胞质粗糙，内有颗粒沉积，１２５，６２５ｍｇ／Ｌ对ＨＳＣ增殖无影响．亚油酸６２５，１２５，２５ｍｇ／Ｌ对ＨＳＣ增殖无明显影响（Ｐ＞００５），但随着浓度增大，５０，１００ｍｇ／Ｌ则促进ＨＳＣ增殖（Ｐ＜００５或Ｐ＜００１）．结论花生四烯酸和亚油酸在一定浓度下可促进肝星状细胞增殖，高浓度对ＨＳＣ有毒性作用。

AIM To study the effect of arachidonic acid and linoleic acid on proliferation of rat hepatic stellate cells (HSC). METHODS HSCs were isolated and cultured from liver of Wistar rats by in situ perfusion with pronase and collagenase, and density gradient centrifugation with nycodenz. MTT colorimetric assay was detected for HSC proliferation. RESULTS Arachidonic acid and linoleic acid had an effect on proliferation of HSC. Arachidonic acid of 25mg/L promoted HSC proliferation ( P <0 01), but 50 and 100mg/L had an inhibitory effect on HSC, and showed cytotoxity on HSC under inverted microscope; 6 25,12 5 and 25mg/L of linoleic acid had no effect on HSC proliferation, but with concentration increasing, 50 and 100mg/L of linoleic acid might promote HSC proliferation. CONCLUSION Arachidonic acid and linoleic acid may promote HSC proliferation, but increased concentration had cytotoxity on HSC. Arachidonic acid and linoleic acid might be associated with fatty liver and hepatic fibrogenesis by lipid peroxidation.