消化系恶性肿瘤患者血清与腹水中细胞因子活性变化

Studies on cytokine activity in sera and ascites of patients with digestive tumor

目的研究消化系恶性肿瘤（ＤＭＴ）患者血清与腹水中内源性ＩＬ２，ＩＬ６，ＩＬ８，ＴＮＦα和ＩＦＮγ的生物学活性．方法应用ＥＬＩＳＡ法检测了１５例ＤＭＴ患者（肝癌１１例，胆总管癌１例，胰腺癌１例，胃癌１例，直肠癌１例）血清与腹水中５种细胞因子活性，并与６例肝硬变（ＬＣ）患者和８例正常成人进行了比较分析．结果ＤＭＴ患者血清ＩＬ２，ＩＬ６的生物学活性显著低于ＬＣ（Ｐ＜００５）；腹水中ＩＬ２，ＩＬ８活性显著低于ＬＣ组（Ｐ＜００１），而ＩＬ６和ＩＦＮγ活性则高于ＬＣ组（Ｐ＜００１，００５）．ＤＭＴ患者血清中ＩＬ６，ＩＬ８活性明显高于正常成人组（Ｐ＜００５）；ＩＬ２，ＩＦＮγ则低于正常成人组，但缺乏显著性．肝癌血清和腹水中ＩＬ２活性显著高于非肝癌组（Ｐ＜００５）；而ＩＬ６活性则相对降低（Ｐ＜００５）．结论恶性肿瘤患者血清中ＩＬ２和ＩＦＮγ活性低于正常人，是ＤＭＴ患者抗肿瘤免疫功能缺陷的标志．

AIM To study the activity of IL 2, IL 6, IL 8, IFNγ and TNFα in the sera and ascites of patients with malignant tumor of digestive system (DMT). METHODS The activity of five cytokines in the sera and ascites of 15 patients with DMT, 6 liver cirrhosis (LC) and 8 normal persons were measured by ELISA. RESULTS The activity of IL 2 and IL 6 in the sera of DMT was significantly lower than that of LC group ( P <0 05). The IL 2 and IL 8 levels in ascites of patients with DMT were significantly lower than that of LC group ( P <0 01). The IL 6 and IFNγ levels in ascites of patients with DMT were higher than that of LC group ( P <0 01 and P <0 05). The activity of IL 6 and IL 8 in the sera of DMT was higher than the normal control group. The activity of IL 2 in sera and ascites of patients with liver cancer was higher than non liver cancer group ( P <0 05); but the IL 6 level was markedly decreased ( P <0 05). CONCLUSION The activity of IL 2 and IFNγ in the sera of patients with DMT is low. IL 6 level is important significance in the prognosis of patients with DMT.