促胃液素及其受体拮抗剂丙谷胺对人胃癌细胞系生长的影响

Effects of gastrin and gastrin receptor antagonist proglumide on gastric cancer line *

目的观察和分析五肽促胃液素ＰＧ及其受体拮抗剂丙谷胺ＰＧＭ对人胃癌细胞系ＭＧＣ生长的影响，为临床应用促胃液素受体拮抗剂协助治疗胃癌提供依据．方法选用５ｍｇ／Ｌ，１０ｍｇ／Ｌ，１５ｍｇ／Ｌ，２０ｍｇ／Ｌ４种浓度的ＰＧ和３０ｍｇ／Ｌ的ＰＧＭ分别作用于体外培养的浓度为２５×１０８／Ｌ的ＭＧＣ，分别培养２４，４８，７２ｈ，于酶标仪上选用波长５４０ｎｍ测定吸光值Ａ，并对数据进行比较分析．结果４种浓度的ＰＧ作用于ＭＧＣ，ＭＧＣ连续３ｄ的生长状态与对照组无明显差异，而ＭＧＣ在ＰＧＭ作用下，其连续３ｄ的平均Ａ值分别为００２９，００４６和００８４，而未被ＰＧＭ作用的ＭＧＣ的平均Ａ值分别是０１０１，０１１５和０１８２，ＭＧＣ在ＰＧＭ作用下生长明显低于对照组（Ｐ＜００５）．结论外源性的ＰＧ对ＭＧＣ无营养促进作用。

AIM To observe and analyze the effect of pentagastrin (PG) and gastrin receptor antagonist proglumide (PGM) on gastric cancer cell line MGC. METHODS A range of PG concentrations ( 5mg/L - 20mg/L ) and a PGM concentration (30mg/L) were separately added to MGC seeded into 96 well plate at a concentration of 2 5×10 8/L. The control was maintained without PG and PGM. After 24, 48 and 72 hours incubation, cell viability was measured by MTT assay (A). RESULTS Cell viability was not significantly different in PG groups as compared with that of control group. The A of MGC with PGM after 24, 48 and 72 hours incubation were 0 029, 0 046 and 0 084. But the A of MGC without PGM was 0 101, 0 115 and 0 182. Cell viability was significantly lower in PGM group as compared with that of control group ( P <0 05). CONCLUSION Exogenous PG has no effect on growth of MGC and PGM can inhibit the growth of MGC.