摘要
目的观察和分析五肽促胃液素PG及其受体拮抗剂丙谷胺PGM对人胃癌细胞系MGC生长的影响,为临床应用促胃液素受体拮抗剂协助治疗胃癌提供依据.方法选用5mg/L,10mg/L,15mg/L,20mg/L4种浓度的PG和30mg/L的PGM分别作用于体外培养的浓度为25×108/L的MGC,分别培养24,48,72h,于酶标仪上选用波长540nm测定吸光值A,并对数据进行比较分析.结果4种浓度的PG作用于MGC,MGC连续3d的生长状态与对照组无明显差异,而MGC在PGM作用下,其连续3d的平均A值分别为0029,0046和0084,而未被PGM作用的MGC的平均A值分别是0101,0115和0182,MGC在PGM作用下生长明显低于对照组(P<005).结论外源性的PG对MGC无营养促进作用。
AIM To observe and analyze the effect of pentagastrin (PG) and gastrin receptor antagonist proglumide (PGM) on gastric cancer cell line MGC. METHODS A range of PG concentrations ( 5mg/L - 20mg/L ) and a PGM concentration (30mg/L) were separately added to MGC seeded into 96 well plate at a concentration of 2 5×10 8/L. The control was maintained without PG and PGM. After 24, 48 and 72 hours incubation, cell viability was measured by MTT assay (A). RESULTS Cell viability was not significantly different in PG groups as compared with that of control group. The A of MGC with PGM after 24, 48 and 72 hours incubation were 0 029, 0 046 and 0 084. But the A of MGC without PGM was 0 101, 0 115 and 0 182. Cell viability was significantly lower in PGM group as compared with that of control group ( P <0 05). CONCLUSION Exogenous PG has no effect on growth of MGC and PGM can inhibit the growth of MGC.
出处
《世界华人消化杂志》
CAS
1999年第1期22-24,共3页
World Chinese Journal of Digestology
基金
湖南省卫生厅基金
关键词
胃肿瘤
病理学
胃泌素
丙谷胺
肿瘤细胞
stomach neoplasms
gastrin
proglumide
tumor cells, cultured