黄蜀葵组织培养的初步研究

A Primary Study of Plant Tissue Culture of Abelmosehus manihot

目的以黄蜀葵子叶和下胚轴为外植体进行组织培养,寻找适宜诱导黄蜀葵子叶和下胚轴愈伤组织发生和分化的激素配比。方法采用不同激素配比MS培养基对黄蜀葵子叶和下胚轴分别培养。结果适宜下胚轴愈伤组织诱导的培养基为MS+IAA0.5mg/L+KT1.0mg/L,MS+IAA0.7mg/L+KT1.0mg/L可诱导愈伤组织产生丛生芽,同时还可诱导下胚轴直接产生幼苗。适宜生根培养基为1/2MS+NAA0.1mg/L,植株粗壮,主根少,须根多。结论本实验初步建立了以黄蜀葵子叶和下胚轴为外植体的组培快繁体系。

Objective To study plant tissue culture of Abelmosehus manihot.Methods The cotyledon and hypocotyl of A.Manihot were cultured in MS media with different proportionings of hormones.Results The inducement of callus in hypocotyl was obtained in the medium MS＋IAA0.5mg/L＋KT1.0mg/L,the inducement of callus to adventitious bud and hypocotyl to seedling were obtained in the medium MS＋IAA0.7mg/L＋KT1.0mg/L.Conclusion A system of plant tissue and rapid propagation based on the explant of the cotyledon and hypocotyl was set up .