四物汤中四种成分的含量测定研究

Study on the Content Determination of Four Components in Siwu Decoction

目的建立四物汤中没食子酸、5-羟甲基糠醛、芍药苷、阿魏酸的含量测定方法。方法采用HPLC法,色谱柱为ZorbaxSB-Aq;流动相为乙腈-0.05%磷酸溶液(梯度洗脱);没食子酸、芍药苷的检测波长为230nm,5-羟甲基糠醛的检测波长为280nm,阿魏酸的检测波长为316nm;流速为1mL·min-1;柱温为40℃。结果没食子酸、5-羟甲基糠醛、芍药苷、阿魏酸的线性范围分别为0.0247～0.4944μg(r=0.9992)、0.01996～0.7984μg(r=0.9999)、0.1181～2.362μg(r=0.9997)、0.1262～0.5048μg(r=0.9996),加样回收率分别为100.22%(RSD=2.06%)、99.83%(RSD=2.70%)、101.37%(RSD=1.59%)、99.89%(RSD=2.29%)。结论四物汤中没食子酸与芍药苷来自白芍,5-羟甲基糠醛来自熟地黄与川芎,阿魏酸来自当归与川芎,为四物汤物质基础的研究提供了一定的参考。

Objective To establish a method for the content determination of gallic acid,5-hydroxymethyl- furfural,peoniflorin and ferulic acid in Siwu Decoction.Methods HPLC with Zorbax SB-Aq column was used,acetonitrile-0.05% phosphoric acid（gradient elution）as a mobile phase.The detection wavelength of four components were 230 nm ,280 nm and 316 nm ,respectively.Flow rate was 1 mL·min-1,and column temperature was 40℃.Results The linear range of four components were 0.024 72~0.494 4 μg（r=0.999 2）,0.019 96~0.798 4 μg（r=0.999 9）,0.1181~2.362μg（r=0.999 7） and 0.126 2~0.504 8 μg（r=0.999 6）with the average recovery 100.22%（RSD=2.06%）,99.83%（RSD=2.70%）,101.37%（RSD=1.59%）and 99.89%（RSD=2.29%）,respectively.Conclusion In Siwu Decoction,gallic acid and peoniflorin come from Radix paeoniae alba,5-hydroxymethyl-furfural comes from Radix rehmanniae praeparata and Rhizoma chuanxiong,and ferulic acid come from Radix angelicae sinensis and Rhizoma chuanxiong ,which provides a reference for the material base of Siwu Decoction.