摘要
利用模糊搜索的方法,在TIGR水稻日本晴基因组数据库(TIGR Rice Genome Annotation-Release5)中识别出565个编码抗病蛋白质的同源序列;利用识别出565个编码抗病蛋白质序列分别与籼稻基因组数据库进行BLASTP联配,共确定320个对应的等位基因。通过在线生物信息学软件,识别了这565个抗病基因的保守结构域、保守模体和DNA序列内转座子元件,其中有14个抗病基因同源序列注释错误。同时绘出了这些基因的基因组分布,并基于这些基因的同源树分析和基因组物理分布,认为基因的原位和远程复制事件产生了抗病基因的现存分布和多样性,其中转座子在复制过程中扮演了重要角色。这些对抗病机制研究和抗病基因进化研究以及抗病基因的转育具有重要意义。
Through a way of fuzzy search, 565 disease resistance protein - encoding genes were identified in the rice genomic sequence of Nipponbare in the rice TIGR database. We identified conserved domains and motifs, and transposable element in these gene protein sequences and DNA sequences. The results showed 14 of them annotations were error. The same time we drew distribution of R genes in the rice genome. Based on homology tree and distribution in chromosomes of these R genes, it was thought that both local and distant genetics duplication events shaped these R genes existing distribution and diversity. Transposable elements played an important role of R gene evolutionary. It was of great significance for the study of the resistant mechanism to disease, evolution and transferring breeding of disease resistance genes.
出处
《生物信息学》
2010年第2期91-97,共7页
Chinese Journal of Bioinformatics
基金
教育部创新团队发展计划项目(IRT0453)
关键词
抗病基因同源序列
基因模体分析
复制事件
转座子元件
基因组
disease resistance gene analogues
gene domains and motifs analysis
duplication event
transposable element
genome
