228株结核分枝杆菌MLVA分型研究

Analysis on the 228 Mycobacterium Tuberculosis with MLVA

目的:通过多位点可变数目串联重复序列(MLVA)分型,了解甘肃省临床分离结核分枝杆菌菌株基因型情况。方法:选择标化的15个VNTR位点,对临床分离菌株DNA进行检测,DNA指纹图谱使用BioNumerics4.5软件进行统计分析,得出聚类分析结果。结果:228株结核分枝杆菌被分为4大基因群,7个主要基因型,分别包含13(5.7%)、3(1.3%)、7(3.1%)、1(0.4%)、171(75.0%)、31(13.6%)、2(0.9%)个菌株;在株水平基因分型上,93(40.8%)株为独立基因型;其余菌株基因型分别包含2～10株结核分枝杆菌,共构成132个基因簇。结论:甘肃省临床分离结核分枝杆菌菌株存在丰富的基因多态性,MLVA方法具有较高的基因分型能力,可以满足结核分枝杆菌株水平DNA分型的需要。甘肃省临床分离结核分枝杆菌菌株主要为北京家族基因型菌株。

Objective：By Multiple locus variable numbers of tandem repeats（MLVA） genotyping,to analyze the genotypes of epidemic Mycobacterium tuberculosis strains in Gansu. Methods：15 standardized MLVA loci were used to type the clinical isolated M. tuberculosis strains of Gansu. Results were analyzed with BioNumerics 4.5. The dendrogram was summarized from the fingerprintings. Results：The result of MLVA showed that 228 strains of M. tuberculosis were classified 4 major genetic groups and 7 main genotypes. The largest genotype included 171（75.0%） strains,the others were 13（5.7%） ,3（1.3%） ,7（3.1%） ,1（0.4%） ,31（13.6%） and 2（0.9%） respectively. Farther particulars,93 strains（40.8%） were belong to unique genotype. The other strains contained 2-10 M. tuberculosis,all 132 geno-clusters. Conclusion：The bacterium strains of M. tuberculosi in Gansu has a rich nucleotide polymorphisms. MLVA is very useful to type the genotype of M. tuberculosi strains. Beijing family genotype strain is the dominant genotype in Gansu.