表皮生长因子及其受体在口腔黏膜上皮异常增生和口腔鳞癌中的表达

The expression of epidermal growth factor and its receptor in oral epithelial dysplasia and oral squamous cell carcinoma

目的探讨表皮生长因子(epidermal growth factor,EGF)及其受体(epidermal growth factor receptor,EGFR)与口腔黏膜癌变的关系。方法①以30例健康成年人唾液样本为对照,通过放射免疫法对12例上皮异常增生患者、40例口腔鳞癌(o-ral squamous cell carcinoma,OSCC)患者唾液EGF含量进行测定分析。②采用免疫组化法检测10例正常口腔黏膜,16例上皮异常增生,30例OSCC上皮组织中EGFR的表达水平。结果①上皮异常增生组唾液EGF含量[(5.12±4.30)μg/L]显著高于口腔鳞癌[(2.35±1.00)μg/L]和正常对照组[(2.18±1.02)μg/L](P<0.01),OSCC和正常对照组无显著性差异。②上皮异常增生组织中EGFR的表达高于正常黏膜(P<0.05)。OSCC中EGFR的表达显著高于正常黏膜(P<0.01)。OSCC组和上皮异常增生组织中EGFR的表达无显著性差异。结论 EGF和EGFR可能参与口腔黏膜癌变的早期阶段。

Objective To investigate the relations between epidermal growth factor（EGF） or epidermal growth factor receptor（ EG- FR） and the carcinogenesis of oral mucosa. Methods （1） The salivary of 12 patients with oral epithelial dysplasia and 40 patients with oral squamous cell carcinoma （OSCC） were measured for EGF concentration by radioimmunoassay（RIA） and the saliva of 30 normal adults as the control. （2）The expression of EGFR was observed in 10 cases of normal oral mucosa, 16 cases of oral epithelial dysplasia and 30 cases of OSCC by immunohistochemical evaluation. Results （1）The salivary EGF levels of oral epithelial dysplasia[ （5.12 ±4. 30） μg/L] were significantly higher than the sEGF levels of OSCC[ （2. 35 ±1.00） μg/L ]and normal oral mucosa[ （2. 18 ±1.02）μg/ L] （P 〈 0. 01 ）. （2）The expression of EGFR in oral epithelial dysplasia was significantly higher than normal oral mucosa （P 〈 0. 05 ）. The expression of EGFR in OSCC was significantly higher than normal oral mucosa（ P 〈0. 01 ）. Conclusions EGF and EGFR may correlate with early malignant transformation stage of oral mucosa.