节节麦对铝的耐性评价

Evaluation the Aluminum Tolerance of Aegilops tauschii Using Hydroponic Nutrient Solutions

为了评价节节麦在普通小麦耐铝方面的应用价值,设置了pH4.50和pH4.50+50μmol/L Al3+以及pH4.50+100μmol/L Al3+3种水培营养液处理45份节节麦。结果表明,再生根伸长量以及耐铝指数在节节麦居群间存在较大的遗传变异。再生根伸长量以pH4.50的最长,pH4.50+100μmol/L Al3+的最短,pH4.50+50μmol/L Al3+的居中。pH4.50+50μmol/L Al3+的耐铝指数变异范围为0.18～0.49,平均值为0.33。pH4.50+100μmol/L Al3+的耐铝指数变异范围为0.09～0.32,平均值为0.18;节节麦材料在50μmol/L Al3+的苏木精染色除As65和As88的染色程度相对较轻(为2级)外,其余染色均较深(为3级),而中国春的染色程度更轻(为1级);综合耐铝指数以及苏木精染色结果认为,所有节节麦都不耐铝,发现耐铝节节麦可能性较小。因此,节节麦可能不是小麦的耐铝抗源之一。

In this study,aluminum tolerance of forty-five Aegilops tauschii were evaluated using three treatments of pH4.50,pH4.50＋50μmol/L Al3＋ and pH4.50 ＋100 μmol/L Al3＋ hydroponic nutrient solutions.The result showed that there were relative remarkable genetic variation in root regenerate lengths （RRL） and aluminum tolerance index （RTI） among accessions.The RRL were the longest at pH4.50,while those in pH4.50＋100μmol/L Al3＋ were the shortest.The RTI varied from 0.18 to 0.49 at pH4.50＋50 μmol/L Al3＋,with an average of 0.33.While RTI varied from 0.09 to 0.32 at pH4.50＋100 μmol/L Al3＋,with an average of 0.18.When the root treated with 50μmol/L Al3＋ and stained with hematoxylin,all of the accessions were stained heavy as scale 3,except for As65 and As88 stained as light as scale 2,while CS stained much lighter than the others and with scale 1.Combined with hematoxylin stain scale and aluminum tolerance index,it was suggested that all of the Aegilops tauschii accessions were sensitive to aluminum.Based on this study,Aegilops tauschii might not one of the best candidates for improving the aluminum tolerance of wheat.