太子参组织培养与快速繁殖研究

Study on Tissue Culture and Rapid Propagation of Pseudostellaria heterophylla

以贵州省施秉县牛大场镇药材基地种植的太子参茎尖、茎段、种根、叶片为材料进行组织培养和快速繁殖研究,结果表明:茎尖和茎段较适合作为组培快繁的材料,初代培养的最适宜培养基为MS+ZT2.0mg/L+NAA0.01～0.08mg/L+3.0%糖+1.2%琼脂;芽诱导率可达90%以上;增殖培养最适培养基为MS+ZT3.0mg/L+NAA0.01mg/L+3.0%糖+1.2%琼脂,最高增殖倍数为10.5;壮苗生根培养基为MS+6-BA3.0mg/L+NAA0.2mg/L+3.0%糖+1.2%琼脂,壮苗培养的同时产生根系和块根,省略了根诱导环节,极大的缩短的培养时间。当石英砂与泥土体积配比为1∶2时移栽存活率最高,达到90%。

The stem-tips、stems、roots and leaves of Pseudostellaria heterophyllalocated in medicine base of Shibing county in Guizhou province were used as explants to establish a rapid propag ation technique system of micropropagation.The results showed that the best explants were stem tips and stems.The induction frequency was above 90% for the stem tips and stems cultured on the medium MS＋ ZT 2.0mg/L＋NAA0.01-0.08mg/L＋3% sugar＋1.2% agar,the differentiation ability of the shoot tip was higher than that of the stem.The suitable medium for adventitious shoot reproduction was MS＋ZT 3.0mg/L＋NAA 0.01mg/L＋3% sugar＋1.2% agar and the proliferation coefficient of adventitious bud reaches 10.The sound seedling medium and the rooting medium of shoots was MS＋6-BA 3.0mg/L＋NAA 0.2mg/L＋3% sugar＋1.2% agar.The root system differentiation was done also during the subculture,which could save the process of root induction and shorten the process of propagation.For the cultivation of vitro plantlet,the suitable ratio of transplanting medium for quartz sand and soil was 1∶2,the survival rate of transplanting reached 90%.